Dubyna V. O., Skrypnykov P. M. Izmailova O. V.

ASSESSMENT OF EFFECT PRODUCED BY ANTIHOMOTOXIC AND NANO-BASED PREPARATIONS ON PERIODONTAL PATHOGENS AND SIGA CONCENTRATION IN THE TREATMENT OF CHRONIC GENERALIZED PERIODONTITIS

---

Show/Download

About the author:

Dubyna V. O., Skrypnykov P. M. Izmailova O. V.

Heading:

DENTISTRY

Type of article:

Scientific article

Annotation:

The modern approach to treating periodontal diseases focuses on controlling periodontal pathogen levels and modulating the immune response. The aim of this study was to qualitatively assess periodontal pathogens in periodontal pockets and measure sIgA concentration in the oral fluid of patients with generalized periodontitis (severity stages I-II) throughout the treatment process. A total of 90 patients with I-II severity of chronic generalized periodontitis (CGP) were included in the study. Group 1 received a treatment complex including the antihomotoxic drug Traumeel C, while Group 2 received a complex with the nano-based preparation Fullerene C60. The presence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, and Treponema denticola was determined via PCR at baseline and in 3, 6, and 12 months of treatment. sIgA levels were measured using an enzyme-linked immunosorbent assay. Before treatment, Tannerella forsythia was the most prevalent pathogen, observed as a mono-infection and in associations in 57.8% and 55.6% of cases, respectively. Porphyromonas gingivalis was detected in 44% (mono-infection) and 40% (associations) of cases; Prevotella intermedia in 42% and 36%; and Treponema denticola in 40% and 37.8%. After 5 days of treatment, 2.75.6% of Group 2 patients showed no detectable periodontal pathogens. Prevotella intermedia, Aggregatibacter actinomycetemcomitans, and Tannerella forsythia were found only as monocultures, in 4.4%, 6.7%, and 6.7% of patients, respectively. After 3 months, the percentage of patients without detectable periodontal pathogens remained stable at 46.7% in Group 1 and 75.6% in Group 2. The baseline sIgA levels in both groups were initially low. Following 3 months of treatment, sIgA concentration increased, with Group 2 patients exhibiting a 27% higher sIgA concentration on average. After 6 months, the spectrum of periodontal pathogens in Group 1 returned to baseline levels. In Group 2, 24.4% of patients continued to show no detectable periodontal pathogens at both the 6-month and 1-year marks. To achieve a stable outcome, it is recommended to re-administer the treatment complex with the Fullerene C60 nanopreparation between 9 and 12 months.

Tags:

Bibliography:

1. Neporada KS, Mykytenko AO, Yankovskyi DS, Shyrobokov VP, Dyment GS. Khronichnyy heneralizovanyy parodontyt yak naslidok porushennya bioplivky biotopu porozhnyny rota. Suchasna Stomatolohiya. 2013;3:22-5. [in Ukrainian].
2. Demkovych A, Kalashnikov D, Hasiuk P, Zubchenko S and Vorobets A () The influence of microbiota on the development and course of inflammatory diseases of periodontal tissues. Front. Oral. Health. 2023;4:1237448. DOI: 10.3389/froh.2023.1237448.
3. Faustova MO, Ananieva MM, Basarab YO, Loban’ GA. Neutrophil bactericidal activity through the stages of placement of different dental implants depending on their chemical composition. Wiad Lek. 2017;70(5):921-924.
4. Yang NY, Zhang Q, Li JL, Yang SH, Shi Q. Progression of periodontal inflammation in adolescents is associated with increased number of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Fusobacterium nucleatum. Int J Paediatr Dent. 2014;24(3):226-33.
5. Naiff PF, Ferraz R, Cunha CF, Orlandi PP, Boechat AL, Bertho AL, Dos-Santos MC. Immunophenotyping in saliva as an alternative approach for evaluation of immunopathogenesis in chronic periodontitis. J Periodontol. 2014;85(5):111-20.
6. Lang NP, Bartold PM. Periodontal health. J Periodontol. 2018;89(1):9-16.
7. Sylenko HM, Skrypnykov PM. Osoblyvosti rozvytku y likuvannya khronichnoho heneralizovanoho parodontytu v osib iz nedostatnistyu sekretornoho imunohlobulinu A. Ukrayinskyy stomatolohichnyy almanakh. 2021;3:70-71. [in Ukrainian].
8. Dubyna VО, Skrypnykov PM. Comparative analysis of applying antihomotoxic drug and nano-based drug in the treatment of chronic generalized periodontitis of stages I-II. Bulletin of problems in biology and medicine. 2024;3(174):401-408.
9. Kaydashev IP, redactor. Metody klinichnykh ta eksperymentalʹnykh doslidzhenʹ v medytsyni. Poltava: VDNZU “UMSA”; 2003. 320 s. [in Ukrainian].
10. Komiyama Y, Kafkova LR, Barasch A, Shah GR, Grbic JT, Novak Z, et al. Origin of galactose-deficient immunoglobulin G in gingival crevicular fluid in periodontitis. J Periodontol. 2014;85(12):1779-85
11. Demkovych A, Bondarenko Y, Shcherba V, Luchynskyi V, Vitkovskyy V, Machogan V. Quercetin effects on adaptive immune response in experimental periodontitis of bacterial-immune genesis. Pharmacia. 2021;68(4):877-882.
12. Skrypnykov PM, Sylenko HM, Sylenko BYU, Khrebor HO, Sydorova AI. Faktory homeostazu porozhnyny rota pry normalʹnomu ta sekretornomu defitsyti IgA. Ukrayinsʹkyy stomatolohichnyy alʹmanakh. 2014;2:18-25. [in Ukrainian].
13. Shulʹzhenko AD. Zmina pokaznykiv m’yazovoho imunitetu rotovoyi porozhnyny u zhinok pry bakterialʹnomu vahinozi. Visnyk Ukrayinsʹkoyi medychnoyi stomatolohichnoyi akademiyi. 2017;4(60):279-282. [in Ukrainian].
14. Predin T, Djurić M, Mirnić J, Gusić I, Nikolić N, Marković D, et al. Clinical and microbiological effects of the initial periodontal therapy. Srp Arh Celok Lek. 2014;142(1-2):10-6.
15. Silva N, Abusleme L, Bravo D, Dutzan N, Garcia-Sesnich J, Vernal R, et al. Host response mechanisms in periodontal diseases. J Appl Oral Sci. 2015;23(3):329-355.

Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 4, 175, 651-659 pages, index UDC 616.31-002.2-031.81-085:[615.015.32+615-022.532]:612.017-07

DOI:

10.29254/2077-4214-2024-4-175-651-659