Shevchuk M. М., Volos L. I.

HISTOLOGICAL ORGANIZATION OF THE PARENCHYMATOUS AND STROMAL-VASCULAR COMPARTMENT OF THE LIVER OF WHITE RATS ON THE 42nd DAY OF EXPERIMENTAL APPLICATION OF HEMP SEED OIL

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About the author:

Shevchuk M. М., Volos L. I.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

Hemp oil, extracted from the seeds of industrial hemp Cannabis sativa L., is widely used in medicine and the food industry. It has a positive effect on the body and on biological and physiological processes due to its ideal ratio of essential polyunsaturated fatty acids. The aim of the study was to evaluate the histological organization of the parenchymal and stromal-vascular components of the liver of rats on the 42nd day of experimental application of hemp seed oil. The study was conducted on 26 sexually mature white male rats weighing 180-230 g in compliance with bioethical standards. The daily dose of hemp seed oil in the experimental group was 0.5 ml/kg, and in the control group – 0.1 ml/kg. The morphological features of the liver were determined, and a morphometric study of the diameter of the vessels of the microcirculatory bed of the liver was performed with statistical data processing. It was found that the structural organization of the parenchymal and stromal-vascular components of the liver on the 42nd day of experimental use of hemp seed oil as a dietary supplement at a dose of 0.5 ml/kg/day was not disturbed, no morphological signs of inflammation and necrotic changes in hepatocytes were found, microvesicular steatosis in <5% of hepatocytes corresponded to grade S0 according to Kleiner and indicated the absence of parenchymal fatty degeneration of the liver. Morphometric analysis of the microcirculatory vessels of the liver on the 42nd day of experimental application of hemp seed oil at a dose of 0.5 ml/kg/day showed no significant difference between the average values of their diameters and the relative values of dilation and fullness in the experimental and control groups and intact animals (p>0.05), indicating the safety of consuming hemp seed oil at a dose of 0.5 ml/kg/day.

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Publication of the article:

«Bulletin of problems biology and medicine», 2025 Issue 4, 179, 359-369 pages, index UDC 616.36–018.2:005:582.635.38]-092.9

DOI:

10.29254/2077-4214-2025-4-179-359-369