Stetsuk Ye. V., Shepitko V. I., Bilash S. M., Pronina O. M., Boruta N. V., Oliinichenko Ya. O.

CHANGES IN THE NUMBER OF CD 163+ MACROPHAGES IN THE TESTICULAR INTERSTITIUM FOLLOWING CHEMICAL CASTRATION WITH TRIPTORELIN

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About the author:

Stetsuk Ye. V., Shepitko V. I., Bilash S. M., Pronina O. M., Boruta N. V., Oliinichenko Ya. O.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

The article examines changes in the quantitative parameters of cells of the monocyte-macrophage lineage in the connective tissue of the testis, identified by CD163+ receptors, during chemical castration with triptorelin over a 365-day period. A progressive increase in the number of CD163+ cells (a 3.5-fold increase over one year) indicates the formation of a stable population of M2-phenotype macrophages. This finding reflects a shift in tissue homeostasis toward an anti-inflammatory response and represents an attempt by the organism to compensate for structural damage to the organ parenchyma caused by hormonal imbalance. The most intensive proliferation and migration of CD163+ macrophages occur during the first 90 days and between days 180 and 270 of the experiment. Prolonged accumulation of M2 macrophages at later stages (days 270-365) may have a dual significance: on the one hand, it limits acute inflammation, while on the other, it creates conditions for excessive connective tissue growth (fibrosis), as this macrophage subtype stimulates fibroblast activity. The plateau of this parameter by day 365 indicates the completion of the phase of active cellular mobilization and the achievement of a threshold level of adaptive remodeling of the macrophage reserve under conditions of chronic triptorelin exposure. Changes in CD163+ expression dynamics may serve as a reliable morphological marker of process chronicity and structural remodeling of the testis during the use of gonadotropin-releasing hormone agonists.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2025 Issue 4, 179, 350-358 pages, index UDC 616.63:477 + 175.67:599.433

DOI:

10.29254/2077-4214-2025-4-179-350-358