Mazur O. Yu.
PATHOMORPHOLOGICAL AND IMMUNOHISTOCHEMICAL FEATURES OF LUNG CHANGES IN OBESITY
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About the author:
Mazur O. Yu.
Heading:
PATHOMORPHOLOGY
Type of article:
Scientific article
Annotation:
Increased secretion of leptin in individuals with excessive amount of adipose tissue can be the basis for the development of comorbid pathology associated with obesity, including lung pathology. Aim of the study – to establish and analyze pathomorphological and immunohistochemical features of lung tissue changes in the autopsy material of deceased obese persons. The total sample of the deceased amounted to 60 deceased. Two research groups were formed: group 1 (control group, n=30) consisted of deceased persons with normal body weight and group 2 (n=30) – of deceased with obesity. The deceased were weighed to measure and evaluate body weight, and the body mass index (BMI) was calculated. The histological and histochemical examination was performed in line with the hematoxylin-eosin staining and Azan trichrome method. Leptin polyclonal antibodies (Thermo Fisher Scientific, Germany) were used to determine leptin expression. The average value (М) and the average (m) error were calculated. Differences in average values were considered significant with a probability level of at least 95% (р<0.05). The average body weight and BMI of the deceased in group 2 were significantly higher than that of the deceased in group 1. According to the results of the pathohistological, histochemical, and immunohistochemical examination of the lung tissue of the deceased in group 1, slight changes in the lung tissue were revealed. In group 2, on the other hand, pronounced pathological changes in the lungs were observed. Pathomorphological changes typical for chronic bronchitis, pulmonary emphysema, focal pneumosclerosis, and the initial manifestation of pulmonary hypertension were found in the obese deceased (group 2). The immunohistochemical study established a moderate and significant expression of leptin in the deceased of group.
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Publication of the article:
«Bulletin of problems biology and medicine», 2023 Issue 2, 169, 410-414 pages, index UDC 616.24:616-056.52]-018-074-078.73
