BIOLOGICAL EFFECTS OF THE LOW-MOLECULAR FRACTION OF THE DORSAL ROOT GANGLIA CRYOEXTRACT ON THE REPRODUCTIVE SYSTEM OF FEMALE RATS

  2. 2023
  3. BIOLOGICAL EFFECTS OF THE LOW-MOLECULAR FRACTION OF THE DORSAL ROOT GANGLIA CRYOEXTRACT ON THE REPRODUCTIVE SYSTEM OF FEMALE RATS

Nesteruk А. V., Legach E. I.

BIOLOGICAL EFFECTS OF THE LOW-MOLECULAR FRACTION OF THE DORSAL ROOT GANGLIA CRYOEXTRACT ON THE REPRODUCTIVE SYSTEM OF FEMALE RATS

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About the author:

Nesteruk А. V., Legach E. I.

Heading:

NANOMEDICINE AND NANOTECHNOLOGY

Type of article:

Scientific article

Annotation:

One of the modern approaches to correcting the reproductive system's function is the use of neurotrophic factors, the source of which can be a fraction of the cryoextract of spinal ganglia. The paper assesses the effect of the low-molecular-weight fraction of the cryoextract of spinal ganglia (<30 kDa) on the ovaries' follicular composition and the rat uterus' contractile activity. The experiments were carried out on white outbred female rats of 6 and 14 months, which corresponds to the reproductive and late reproductive age for this species of animals. The cryoextract was obtained from the spinal ganglia of neonatal piglets. The cryoextract fraction with a molecular weight of up to 30 kDa was obtained by the method of ultrafiltration using a polyethersulfone membrane. The total cryoextract and its low molecular weight fraction were administered intraperitoneally to rats for 9 days. After the end of the experiment, a histological examination of the ovarian follicles and a study of the contractile activity of the uterus by the organ bath method were performed. It was established that the total cryoextract of spinal ganglia and its low-molecular fraction differ in their biological effect on the reproductive system of female rats of different ages. The fraction of cryoextract of spinal ganglia exerts an activating effect on the follicular profile of the ovaries of rats of reproductive age, which is expressed in a decrease in the number of follicles of hormone-independent stages of development. At the same time, introducing the fraction decreases the force of isometric contraction of the uterus in rats, which may be associated with physiological changes in its innervation.

Tags:

contractile activity, dorsal root ganglia cryoextract, neurotrophic factors, ovary, uterus

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Publication of the article:

«Bulletin of problems biology and medicine», 2023 Issue 2, 169, 402-409 pages, index UDC 615.361:612.622/.621.5

DOI:

10.29254/2077-4214-2023-2-169-402-409

2023 Issue 2, 169
contractile activity dorsal root ganglia cryoextract neurotrophic factors ovary uterus
29.06.2023
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