Hnatyuk M. S., Dovgyy B. I., Tatarchuk L. V., Stets N. Ya., Jasinovskyi O. B.

QUANTITATIVE MORPHOLOGICAL ASSESSMENT OF STRUCTURAL CHANGES OF MYOCYTES OF THE MUSCULAR COAT OF THE URINARY BLADDER AT CONDITIONS OF LONG-TERM POSTRESECTION PORTAL HYPERTENSION

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Hnatyuk M. S., Dovgyy B. I., Tatarchuk L. V., Stets N. Ya., Jasinovskyi O. B.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

The urinary bladder is an organ of the urinary system that is often involved in pathological processes affecting the urethra, ureters, kidneys, and prostate gland. Some researchers argue that haemodynamic disturbances in the large and small circulatory systems, as well as the portal system, also lead to structural and functional changes in the urinary bladder. To date, there are several controversial and unclear issues in the pathogenesis of various blad- der lesions, among which the morphogenesis of the muscular coat of the bladder in cases of portal system blood flow disorders remains unexplored. Therefore, a detailed, comprehensive study of the muscular coat of the urinary bladder deserves attention. Isolated smooth myocytes of the muscular coat of the urinary bladder of three groups of Vietnamese male pigs were morphometrically studied: 5 control animals were included in the first group, 11 pigs with post-resection portal hypertension were included in the second group, and 6 animals with a combination of portal hypertension and mul- tiple organ failure were included in the third group. Isolated smooth muscle cells of the muscular coat were obtained by alkaline cell dissociation, followed by the preparation of smears stained with azure-eosin. The cells were divided into small, medium and large according to their size, and the volumes of myocytes, nuclei, nuclear-myocyte ratios of the studied cells, and the volumes of their damage were also determined. The percentages of cells of different sizes were counted. The quantitative values were statistically processed. It has been established that resection of the left and right lateral lobes of the liver in male pigs of the Vietnam- ese breed led to post-resection portal hypertension. It was also found that postoperative hypertension in the portal system is accompanied by venous congestion of the urinary bladder, ischaemia, hypoxia and atrophy of myocytes, disruption of nuclear-myocyte relations in them, an increase in the volume of damaged myocytes, a decrease in the number of small and medium cells, and an increase in the number of large myocytes. It was established that the most pronounced quantitative morphological changes were observed in small smooth muscle cells of the urinary bladder when post-resection portal hypertension was combined with multiple organ failure.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», Issue 2,177, 422-429 pages, index UDC 616.136.4-003.93-06:616.36-089.87

DOI:

10.29254/2077-4214-2025-2-177-422-429