Pshychenko V. V., Cherno V. S., Chebotar L. D., Naidich O. V., Petrova O. I.
PROOXIDANT STATE OF RAT LUNG TISSUE UNDER CONDITIONS OF CADMIUM CHLORIDE INTOXICATION AND CORRECTION WITH MELATONIN
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About the author:
Pshychenko V. V., Cherno V. S., Chebotar L. D., Naidich O. V., Petrova O. I.
Heading:
CLINICAL AND EXPERIMENTAL MEDICINE
Type of article:
Scientific article
Annotation:
Heavy metals are the most common environmental pollutants and pose a serious threat to public health. Cadmium compounds are particularly dangerous because they have a long half-life in the body. Today, in the conditions of military operations taking place on the territory of Ukraine, cadmium enters the body in quantities that can significantly exceed the maximum permissible standards. Accumulating in the body, cadmium stimulates the development of oxidative stress and can lead to diseases of vital organs, including the lungs, a decrease in their function, and death. Therefore, a pressing issue is the search for effective ways to correct pathological changes caused by the toxic effects of cadmium and oxidative stress. To correct disorders that arise as a result of oxidative stress, drugs with antioxidant properties are used. One of the antioxidants that absorbs free radicals well and has a wide range of therapeutic effects is the pineal gland hormone melatonin. The aim of the work was to study the features of changes in the functional state of the prooxidant system of the lungs of rats under conditions of cadmium chloride intoxication and to substantiate the possibility of their correction with melatonin. The studies were conducted on 36 sexually mature white male Wistar rats, which were divided into six groups – a control group and five experimental groups. Animals of the first (control) group were kept in standard vivarium conditions without the influence of additional factors. Animals of the second, third, fourth, and fifth groups were intraperitoneally administered a 0.029% aqueous solution of cadmium chloride at a rate of 1.2 mg/kg of animal body weight, which would cause lung damage. For the purpose of comparative analysis of biochemical parameters, rats from the experimental groups were withdrawn from the experiment at different times during the study. Metabolic changes were examined on the 7th, 14th, 21st, and 28th days of the study. Rats of the sixth group were administered 1.2 mg/kg of body weight of the animal with a solution of cadmium chloride for 28 days and, in order to correct pathological changes caused by cadmium, melatonin was administered intraperitoneally at a dose of 3.0 mg/kg of body weight for 10 days (from the 19th to the 28th day of the study). It was found that the administration of cadmium chloride was accompanied by an intensification of lipoperoxidation processes, which was manifested by an increase in the content of diene conjugates and malondialdehyde in lung homogenates at all study periods. The introduction of exogenous melatonin contributed to a decrease in the content of primary and secondary products of lipid peroxidation, which indicates a slowdown in free radical processes. Therefore, melatonin is a potentially useful drug for reducing the manifestations of oxidative stress caused by cadmium chloride exposure in lung tissue.
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Bibliography:
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Publication of the article:
«Bulletin of problems biology and medicine», 2025 Issue 1, 176, 297-305 pages, index UDC 615.099:599.323.452:591.424
