Paydarkina A. P., Kush O. G.

MORPHOFUNCTIONAL CHANGES OF THE PERITONEUM AND ITS STRUCTURES WITH ADHESION DISEASE

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About the author:

Paydarkina A. P., Kush O. G.

Heading:

BIOLOGY

Type of article:

Scientific article

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The study of the pathogenesis of adhesions and the role of lymphoid clusters FALC in adhesion formation remains an urgent problem in modern biology and medicine. Bowel obstruction occupies a special place among all other potential consequences of adhesive disease, which emphasizes the medical and social significance of this problem. The present work presents the peculiarities of the distribution and number of lymphocytes in the small intestinal mesentery in adhesive disease compared to the norm in rats. Purpose: to determine the peculiarities of the morphofunctional state of peritoneal tissues and fat-associated lymphoid clusters (FALC) in rats in normal and adhesive disease. Object and Methods: dissection, macroscopic, microscopic, histological (making film preparations), hematoxylin and eosin staining, mathematical (morphometric grid - the number of immunocompetent cells per standard area of 1000 μm2 was counted), planimetric (the plane of the small intestine mesentery and the area changed by adhesive processes were calculated), statistical by Student. In the mesentery of the small intestine, fat-associated lymphoid clusters FALC, represented by diffusely located lymphocytes of small, medium and large diameter and plasma cells, are normally observed. After reproduction of the experimental form of adhesive disease, gradual changes in the tissues of the mesentery of the small intestine were observed: a decrease in mobility and granularity of the tissue on day 7 of observation, thickening and density of connective tissue on day 14, as well as the appearance of granular formations with solid conglomerates of heterogeneous structure in the tissue on day 21 of observation. The influence of adhesion formation processes on the structure of lymphoid tissue was also observed, and the presence of lymphocyte clusters in certain areas of the mesentery was observed on day 7 of the study. On day 14, the number of lymphocytes per unit area increased by 44%, and on day 21, by 47% compared to day 7 of observation.

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Bibliography:

1. Hu Q, Xia X, Kang X, Song P, Liu Z, Wang M, et al. A review of physiological and cellular mechanisms underlying fibrotic postoperative adhesion. Int J Biol Sci. 2021;17(1):298-306.
2. Domoratsky AE, Krylyuk VO, Pavlenko AI, Pyatkovska NV, Sozansky VV. Adhesive disease: the current state of the problem. Acute and urgent conditions in the doctor’s practice. 2016;3-4:19-26.
3. Mandziy OV, Milyevsʹkyy AYE. Dubchak Е. Vidnovlennya reproduktyvnoho potentsialu u zhinok z bezplidnistyu ta khronichnym tazovym bolem. Zdorovʺya zhinky. 2012;10:162-165. [in Ukrainian].
4. Veselyy SV, Klimansʹkyy RP, Nhuyen N. Suchasni tendentsiyi u profilaktytsi ta likuvanni spaykovoyi kyshkovoyi neprokhidnosti u ditey (ohlyad literatury). Khirurhiya dytyachoho viku. 2017;4:46-52. [in Ukrainian].
5. Bazhora YUI, Petrashevych YUV. Mekhanizmy makromolekulyarnykh vzayemodiy u systemnomu homeostazi pry formuvanni pervynnoyi imunnoyi vidpovidi v eksperymenti. Bukov. med. visn. 2001;5(3):162-167. [in Ukrainian].
6. Copin P, Ronot M, Nuzzo A, Maggiori L, Bouhnik Y, Corcos O. Inter–reader agreement of CT features of acute mesenteric ischemia. Eur J Radiol. 2018;105:87-95.
7. Horalʹskyy LP, Khomych VT, Kononsʹkyy OI. Osnovy histolohichnoyi tekhniky i morfofunktsionalʹni metody doslidzhenʹ u normi ta pry patolohiyi. Navchalʹnyy posibnyk. Zhytomyr: Polissya. 2005;6:70-71. [in Ukrainian].
8. Paydarkina AP, Kush OG. Doslidzhennya morfolohichnykh osoblyvostey ocherevyny bilykh shchuriv y metodyka yiyi zaboru. Morphologia. 2023;17(3):163-168.
9. Jayanth ST, Pulimood A, Abraham D, Rajaram A, Paul MJ, Nair A. A randomized controlled experimental study comparing chitosan coated polypropylene mesh and ProceedTM mesh for abdominal wall defect closure. Ann Med Surg. 2015;4(4):388-94.
10. Voloshyn MA, Chaykovskyy YUB, Kushch OH. Osnovy imunolohiyi ta imunomorfolohiyi. Zaporizhzhya-Kyyiv: Zaporiz. derzh. med. un-t, Nats. med. un-t im. O.O. Bohomolʹtsya; 2010. 170 s. [in Ukrainian].
11. Felix MD. Observation on the surface cells of the mouse omentum as studied with the Phasecontrast and Electron Microscopes. Journal of the National Cancer Institute. 1961;27:713-745.
12. Khashchuk VS. Mekhanizmy rozvytku spaykovoho protsesu ocherevynnoyi porozhnyny (ohlyad literatury). Klinichna ta eksperymentalʹna patolohiya 2021;20.4(78):137-145. [in Ukrainian].
13. Bodnar OB. Likuvannya ta profilaktyka spaykovoyi khvoroby u ditey pislya perenesenykh hostrykh khirurhichnykh zakhvoryuvanʹ orhaniv cherevnoyi porozhnyny. Donetsʹk; 2003. 17 s. [in Ukrainian].
14. Tarhan OR, Barut RI. Pentoxifylline, a methyl xanthine derivative, reduces peritoneal adhesions and increases peritoneal fibrinolysis in rats. Tohoku J. Exp. Med. 2006;209(3):249-55.
15. Chegini N. Peritoneal molecular environment, adhesion formation and clinical implication. Front. Biosci. 2002;7:91-115.
16. Delibegovic S, Koluh A, Cickusic E, Katica M, Mustedanagic J, Krupic F. Formation of adhesion after intraperitoneal application of TiMesh: experimental study on a rodent model. Acta. Chir. Belg. 2016;116(5):293-300.
17. Geng HX. Control and prevention of peritoneal adhesions in gynecologic surgery. Practice Committee of the American Society for Reproductive Medicine. Fertil. Steril. 2006;86(5):1-5.
18. Braun KM, Diamond MP. The biology of adhesion formation in the peritoneal cavity. Seminars in Pediatric Surgery. 2014;23(6):336-343.
19. Fortin CN, Saed GM, Diamond MP. Predisposing factors to post-operative adhesion development. Human Reproduction Update. 2015;21(4):536-551.

Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 1, 172, 97-106 pages, index UDC 57.085:[599.323.452:591.438]

DOI:

10.29254/2077-4214-2024-1-172-97-106