Chernobai N. A., Bulavina V. S., Rudnieva K. E.
IMPROVING DNA PRESERVATION TECHNIQUES FOR FORENSIC AND SCIENTIFIC APPLICATIONS
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About the author:
Chernobai N. A., Bulavina V. S., Rudnieva K. E.
Heading:
METHODS AND METHODOLOGIES
Type of article:
Scientific article
Annotation:
The resolution of pressing issues in forensic science, medicine, and other scientific research requires the development of methods for long-term storage of DNA samples. This issue is becoming increasingly important against the backdrop of advancements in cutting-edge technologies that enable the efficient preservation of genetic information and its application in various fields. The aim of this study was to explore new, more effective methods for obtaining and storing DNA samples from human touch DNA traces. Control human DNA from the "GlobalFiler™" test kit (USA) at a final concentration of 0.5 ng/μl was used as the DNA matrix. Genetic material was collected from sweat and grease secretions on fingertip ridges. DNA swabs from human fingerprint residues were stored at various temperatures ranging from 20°C to -196°C for 30 days. The results demonstrated that storage at room temperature (20°C) caused significant DNA degradation. Lowering the storage temperature increased DNA integrity preservation after thawing. The use of a modified buffer (10 mM Tris (hydroxymethyl) aminomethane, 0.5% v/v Tween 20, 50 mM KCl, 0.1 mM EDTA (ethylenediaminetetraacetic acid), 5% glycerol, 0.1% NaN3 (sodium azide), sterile deionized water) during DNA extraction proved more effective compared to deionized water, reducing DNA loss even at room temperature. The conducted research underscores the need to improve DNA preservation methods in forensic and research practices and highlights the effectiveness of cryopreservation in extending the shelf life of DNA samples
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Bibliography:
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Publication of the article:
«Bulletin of problems biology and medicine», 2024 Issue 4, 175, 510-517 pages, index UDC 577.15:343.9
