Khanenko O. B., Popovych Yu. I.

EFFECTS OF EXPERIMENTAL STREPTOZOTOCIN-INDUCED DIABETES AND ITS CORRECTION ON THE MORPHOMETRIC PARAMETERS OF LYMPHOCYTES IN THE T-CELL ZONE OF THE SPLEEN

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About the author:

Khanenko O. B., Popovych Yu. I.

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PROSPECTS FOR THE DEVELOPMENT OF MORPHOLOGICAL AND CLINICAL RESEARCH

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Scientific article

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Morphometric parameters of splenic lymphocytes were investigated in the late stages of streptozotocin-induced diabetes mellitus and under the influence of drug correction with insulin and dapagliflozin. Male rats were divided into five groups: intact, control, streptozotocin-induced diabetes mellitus, diabetes with insulin treatment, and diabetes with insulin and dapagliflozin treatment. Spleen samples were collected on days 42 and 56 of the experiment. Histological sections were stained with hematoxylin and eosin (H&E). Morphometric analysis was performed using the ImageJ software. K-means clustering was used to classify the lymphocyte populations based on their morphometric characteristics. In diabetes mellitus, the proportion of small lymphocytes decreased significantly, whereas the proportion of medium- and large-sized lymphocytes increased. Morphometric analysis revealed a significant decrease in cell and nuclear profile areas, along with notable cell deformation. Insulin correction partially restored the lymphocyte content of different sizes, whereas its combined application with dapagliflozin yielded better results. Insulin correction partially restored the nuclear-cytoplasmic ratio, which was more pronounced when combined with dapagliflozin, with morphometric parameters approaching those of the control group. The most significant changes were observed in the large lymphocyte population. Combined correction with insulin and dapagliflozin had the most notable restorative effect. The obtained results indicate that the correction of metabolic disorders can partially prevent or slow down morpho-functional changes in splenic lymphocytes during the late stages of experimental diabetes mellitus (DM).

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2025 Issue 4, 179, addition, 72-74 pages, index UDC 616.411-018:616.379-008.64-092.9

DOI:

10.29254/2523-4110-2025-4-179/addition-72-74