Sulym H. А., Lyndin M. S., Sikora K. O., Romaniuk A. M.

DYNAMICS OF SKIN RECOVERY IN RATS AFTER ULTRAVIOLET EXPOSURE

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Sulym H. А., Lyndin M. S., Sikora K. O., Romaniuk A. M.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

Ultraviolet (UV) radiation is not only a common natural pathogenic factor that is a significant factor in the etiology of various skin diseases, but it can also be used in treating skin diseases. Thus, for the treatment of such common dermatoses as vitiligo, psoriasis, atopic dermatitis, monochromatic UV radiation with the maximum power of the light flux with a wavelength of 311 nm - narrow-band UV radiation (UV-B) is used. The study aimed to investigate the morphological and functional changes occurring in the epidermis of rats exposed to UV radiation. It also evaluated the dynamics of recovery, its speed and features, pathological changes of the epidermis, and its thickness, as a sign of the persistence of pathological processes. The experiment was based on the simulation of the therapy of skin diseases with narrow-band UV radiation. The study's biological material was collected from 30 animals, 6 individuals per group: intact group (control group), on the 31st (one day after the last session of UV irradiation), 45th, 61st and 121st days experiment. The morphological picture was compared with the native structure of the skin of rats in the control group. Peak morphofunctional changes were present on the 31st day of the experiment - after the end of UV irradiation. In this period, together with the pathological picture, statistical differences in the thickness of the epidermis from the control group were most clearly manifested (p=0.002). At the next stages of the experiment, the pathological changes of the epidermis spread in the direction from the Malpighian layer to the entire height of the epidermis (dystrophy of the cells of the basal layer, intensification of the spinous layer, disruption of the organization of the granules of the granular layer, etc.) and further to the focus of morphological abnormalities mainly in the upper layers of the epidermis (phenomena of hyperkeratosis). The morphometry of the average thickness of the epidermis showed a statistically significant deviation from the norm in the direction of increase during the entire experiment period (121 days). Pathological signs were also preserved. At the same time, epidermal thickness indicators tended to normalize over time. The predicted normalization of the indicator of the average thickness of the epidermis was supposed to occur on the 231st day (according to linear regression). The epidermis shows a number of pathological signs after a course of UV radiation. The average thickness of the epidermis is an indicator among others that can be objectively measured and shows a statistically significant increase compared to the normal indicator by day 121 (р=0.009). According to the calculated forecast, it can normalize only on the 231st day of potential observation. Thus, the average thickness measurement of the epidermis can be used to evaluate morphofunctional changes in the epidermis.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 1, 172, 435-443 pages, index UDC 616.5-092.9:615.84

DOI:

10.29254/2077-4214-2024-1-172-435-443