Cheliy A. O., Pantus A. V., Rozhko M. M.

COMPARATIVE ANALYSIS OF THE RESULTS OF COMBINED MINERALISED AND DEMINERALISED FIBRIN-BONE SCAFFOLDS USE IN SINUS-LIFT TECHNIQUE

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Cheliy A. O., Pantus A. V., Rozhko M. M.

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DENTISTRY

Type of article:

Scientific article

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The aim of the study was to compare the effectiveness of mineralised and demineralised fibrin-bone scaffolds formed using the author's method in the sinus lift technique. Sixty patients (mean age, 30.0±0.5 years) with alveolar process atrophy were divided into two groups, in which the authors' protocol for preparing fibrin-bone scaffolds using a titanium filter was employed. Group I (n=30) included patients who underwent the sinus lift technique using a known surgical approach with bone-plastic material in the form of mineralised granules. Group II (n=30) included patients who underwent sinus lift surgery using a well-established surgical approach with bone-plastic material in the form of demineralised granules and an insulating collagen membrane. The structure of the resulting bone-fibrin scaffold was analysed using a scanning electron microscope and X-ray methods. The effectiveness of the author's technique was evaluated based on electron microscopic, clinical and radiological indicators. Statistical analysis of quantitative data was performed using Microsoft Excel 2019 software. It was established that the fibrin component introduced into the graft is the basis for the formation of closed general plates around the allograft in the process of neo-osteogenesis after transplantation of the fibrin-bone scaffold into the surgical field. The nature of the allograft surface, which is well visualised by X-ray and scanning electron microscopy, plays a crucial role in the mechanism of such remodelling. It has been shown that fine-looped surfaces are most suitable for the development of contact and distant osteogenesis processes. Conclusions: 1) a stable combined scaffold has a pronounced osteoconductive and osteoinductive effect, which is reflected in the restoration of bone tissue throughout the entire augmentation area in the second group of patients; 2) X-ray studies indicated the formation of bone tissue close to intact bone when using demineralised granules.

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Bibliography:

1. Kim BJ, Kwon TK, Baek HS, Hwang DS, Kim CH, Chung IK, et al. A comparative study of the effectiveness of sinus bone grafting with re- combinant human bone morphogenetic protein 2-coated tricalcium phosphate and platelet-rich fibrin-mixed tricalcium phosphate in rabbits. Oral Surg Oral Med Oral Pathol Oral Radiol. 2012;113(5):583-92. DOI: 10.1016/j.tripleo.2011.04.029.
2. Amam MA, Abdo A, Alnour A, Amam A, Jaafo MH. Comparison of calcium sulfate and tricalcium phosphate in bone grafting after sinus lifting for dental implantation: A randomized controlled trial. Dent Med Probl. 2023;60(2):239-246. DOI: 10.17219/dmp/151983.
3. Okada T, Kanai T, Tachikawa N, Munakata M, Kasugai S. Long-term radiographic assessment of maxillary sinus floor augmentation using beta-tricalcium phosphate: Analysis by cone-beam computed tomography. Int J Implant Dent. 2016;2(1):8. DOI: 10.1186/s40729-016-0042-6.
4. Oba Y, Tachikawa N, Munakata M, Okada T, Kasugai S. Evaluation of maxillary sinus floor augmentation with the crestal approach and beta-tricalcium phosphate: A cone-beam computed tomography 3- to 9-year follow-up. Int J Implant Dent. 2020;6(1):27. DOI: 10.1186/s40729-020-00225-7.
5. Avetikov DS, Stavytskyy SA, Lokes KP, Yatsenko IV. Otsinka efektyvnosti auhmentatsiyi alveolyarnoho hrebnya na etapi pidhotovky do dentalnoyi implantatsiyi. Visnyk problem biolohiyi i medytsyny. 2016;1(131):240-2. [in Ukrainian].
6. Pavlenko AV, Dmytryeva ÉA, Luzyn VY. Gistologicheskoye stroyeniye regenerata pri zapolnenii kostnogo defekta materialami easygraft i trikal’tsiyfosfatom. Morfologiya. 2011;5 (2):49-54.
7. Pavlenko OV, Dmytriyeva YEO. Morfolohichni osnovy vyboru kistkovo plastychnykh materialiv u parodontolohiy. Morfologiya. 2011;5(1):5- 12. [in Ukrainian].
8. Berwig KH, Baldasso C, Dettmer A. Production and characterization of poly(3-hydroxybutyrate) generated by Alcaligenes latus using lactose and whey after acid protein precipitation process. Bioresour. Technol. 2016;218:31-7.
9. Hapach LA, VanderBurgh JA, Miller JP, Reinhart-King CA. Manipulation of in vitro collagen matrix architecture for scaffolds of improved physiological relevance. Physical Biology. 2015;12(6):061002. DOI: 10.1088/1478-3975/12/6/061002.
10. Pantus AV, Rozhko MM, Paliychuk VI, Kovalchuk NY, Melnyk NS. Microstructure of biopolymer micro-fibrous scaffold and its influence on the ability to retain medicines and tissue regeneration. Georgian medical news. 2023;3(336):37-44.
11. Crisci A, Lombardi D, Serra E, Lombardi G, Cardillo F, Crisci M. Standardized protocol proposed for clinical use of L-PRF and the use of L-PRF Wound Box®. J Unexplored Med Data. 2017;2:77-87. DOI: 10.20517/2572-8180.2017.17.
12. Kubesch A, Barbeck M, Al-Maawi S, Orlowska A, Booms P, Sader R, et al. A low-speed centrifugation concept leads to cell accumulation and vascularization of solid platelet-rich fibrin: An experimental study in vivo. Platelets. 2019;30(3):329-340. DOI: 10.1080/09537104.2018.1445835.
13. Lee HM, Shen EC, Shen JT, Fu E, Chiu HC, Hsia YJ. Tensile strength, growth factor content and proliferation activities for two platelet concentrates of platelet-rich fibrin and concentrated growth factor. J. Dent. Sci. 2020;15(2):141-146. DOI: 10.1016/j.jds.2020.03.011.
14. Li W, Sigley J, Pieters M, Helms C, Nagaswami C, Weisel JW, et al. Fibrin Fiber Stiffness Is Strongly Affected by Fiber Diameter, but Not by Fibrinogen Glycation. Biophys. J. 2016;110(6):1400-1410. DOI: 10.1016/j.bpj.2016.02.021.
15. McLellan J, Plevin S. Temporal release of growth factors from platelet- rich fibrin (PRF) and platelet-rich plasma (PRP) in the horse: a comparative in vitro analysis. International Journal of Applied Research in Veterinary Medicine. 2014;12(1):48-57.
16. Caymaz G, Uyanik O. Comparison of the effect of advanced platelet-rich fibrin and leukocyte- and platelet-rich fibrin on outcomes after removal of impacted mandibular third molar: A randomized split-mouth study. Niger. J. Clin. Pract. 2019;22(4):546-552. DOI: 10.4103/njcp.njcp_473_18.
17. Canellas JVDS, Medeiros PJD, Figueredo CMDS, Fischer RG, Ritto FG. Platelet-richfibrin in oral surgical procedures: A systematic review and meta-analysis. Int. J. Oral Maxillofac. Surg. 2019;48(3):395-414. DOI: 10.1016/j.ijom.2018.07.007.
18. Caruana A, Savina D, Macedo JP, Soares SC. From Platelet- Rich Plasma to Advanced Platelet-Rich Fibrin: Biological Achievements and Clinical Advances in Modern Surgery. Eur. J. Dent. 2019;13(2):280-286. DOI: 10.1055/s-0039-1696585.
19. Crisci A, Manfredi S, Crisci M. Fibrinrich in Leukocyte- Platelets (L-PRF) and Injectable Fibrin Rich Platelets (I-PRF), two opportunity in regenerative surgery: Review of the sciences and literature. IOSR Journal of Dental and Medical Sciences (IOSR-JDMS). 2019;18:66-79.
20. Choukroun J, Ghanaati S. Reduction of relative centrifugation force within injectable platelet-rich-fibrin (PRF) concentrates advances patients’ own inflammatory cells, platelets and growth factors: The first introduction to the low speed centrifugation concept. Eur. J. Trauma Emerg. Surg. 2018;44(1):87/-5. DOI: 10.1007/s00068-017-0767-9.
21. Siawasch SAM, Andrade C, Castro AB, Temmerman A, Quirynen M. Impact of local and systemic antimicrobials on leukocyte- and platelet rich fibrin: an in vitro study. Sci Rep. 2022;12:2710. DOI: 10.1038/s41598-022-06473-4.
22. Crisci A, Barillaro MC, Lepore G, Cardillo F. L-PRF Membrane (FibrinRich in Platelets and Leukocytes) and Its Derivatives (A-PRF, i- PRF) are Help ful as a Basis of Stem Cells in Regenerative Injury Treatment: Trial Work on the Horse. International Blood Research & Reviews. 2019;10(2):1-14.
23. Crisci A, Benincasa G, Crisci M, Crisci F. Leukocyte Platelet-Rich Fibrin (L-PRF), a new biomembrane useful in tissue repair: basic science and literature review. Biointerface Research in Applied Chemistry. 2018;8(5):3635-43.
24. De Almeida NCPM, Dos Santos NBM, Completo AMG, De Oliveira FGV. Tensile strength assay comparing the resistance between two different autologous platelet concentrates (leucocyte-platelet rich fibrin versus advanced-plateletrichfibrin): A pilotstudy. Int. J. Implant Dent. 2021;7(1):1. DOI: 10.1186/s40729-020-00284-w.
25. El Bagdadi K, Kubesch A, Yu X, Al-Maawi S, Orlowska A, Dias A, et al. Reduction of relative centrifugal forces increases growth factor release within solid platelet- rich fibrin (PRF)-based matrices: a proof of concept of LSCC (low speed centrifugation concept). European Journal of Trauma and Emergency Surgery. 2019;45(3):467-79.
26. Humenyuk RA. Zasoby dlya optymizatsiyi osteohenezu v stomatolohiyi: oblast zastosuvannya, aktualnist problemy i perspektyvy rozrobok i vprovadzhennya novykh preparativ. K.: Zdorovya; 2021. 152 s. [in Ukrainian].

Publication of the article:

«Bulletin of problems biology and medicine», 2025 Issue 3,178, 556-568 pages, index UDC 616-071+616,314-007+616.716

DOI:

10.29254/2077-4214-2025-3-178-556-568