Prykhodko S. O., Shkolnikov V. S., Aleksyeyenko N.S., Zalevskiy L. L., Danylevych V. P.
CHARACTERISTICS OF THE EXPRESSION OF MONOCLONAL ANTIBODIES DURING THE PERIOD OF THE MARGINAL AND THORACIC NUCLEI FORMATION
Show/Download
About the author:
Prykhodko S. O., Shkolnikov V. S., Aleksyeyenko N.S., Zalevskiy L. L., Danylevych V. P.
Heading:
MORPHOLOGY
Type of article:
Scientific article
Annotation:
Embryology is one of the most relevant and popular areas in medicine. Without exaggeration, it is one of the most important branches of medicine and biology. Thanks to embryology, we can better understand the development processes of organisms, the causes of birth defects, and the possibilities of treating infertility. Immunohistochemical methods have significantly improved the understanding of the mechanisms that control the differentiation of neural stem cells into neurons and glial cells, as well as the processes of targeted migration. The aim of the study - to characterize the expression of monoclonal antibodies during the development of marginal and thoracic nuclei. 134 preparations of human embryos and fetuses aged from 6-7 to 39-40 weeks of intrauterine development were used, which developed in the womb without the influence of harmful factors, both internal and external environment. The research was conducted with the mothers’ written consent and in compliance with all ethical and deontological norms. To perform immunohistochemical methods, monoclonal antibodies from the company “DacoCytomation” (Denmark): vimentin, synaptophysin, Ki-67, CDX2, S-100 were used. As a result of the study, it became clear that the expression of monoclonal antibodies is completely dependent on the processes inherent in each of the periods of intrauterine development. Before the end of the period of intrauterine development, the expression of Ki-67 is weak, and vimentin and CDX-2 are completely absent. Instead, the expression of S-100 and synaptophysin in the studied nuclei is alternately relatively strong.
Tags:
Bibliography:
- Arulkandarajah KH, Osterstock G, Lafont A, Le Corronc H, Escalas N, Corsini S, et al. Neuroepithelial progenitors generate and propagate non-neuronal action potentials across the spinal cord. Current Biology. 2021;31(20):4584-4595. DOI: 10.1016/j.cub.2021.08.019.
- Bardoni R. Serotonergic Modulation of Nociceptive Circuits in Spinal Cord Dorsal Horn. Curr Neuropharmacol. 2019;17(12):1133-1145. DOI: 10.2174/1570159X17666191001123900.
- Kuzmicz-Kowalska К, Kicheva A. Regulation of size and scale in vertebrate spinal cord development. Developmental Biology. 2021;10(3):383. DOI: 10.1002/wdev.383.
- Srivastava G. Langman’s medical embryology by T Sadler. Era’s Journal of Medical Research. 2018;5(1):86-87. DOI: 10.24041/ejmr2018.70.
- Zalevskiy LL, Shkolnikov VS, Prykhodko SO. Histostructural organization of the cerebellum of human fetuses for 8-9 weeks of prenatal development. Reports of Morphology. 2019;25(3):45-51. DOI: 10.31393/morphology-journal-2019-25(3)-08.
- Lavezzi AM, Alfonsi G, Pusiol T. De-creased argyrophilic nucleolar organiser region (AgNOR) expression in Purkinje cells: first signal of neuronal damage in sudden fetal and infant death. Journal of Clinical Pathology. 2016;69(1):58-63. DOI: 10.1136/jclin-path-2015-202961.
- Vernygorodskyi SV, Shkolnikov VS, Prykhodko SO. The morphology and neuronal-glial correlations of the spinal cord posterior horns of human embryos. International medicine. 2021;3(3):93-100.
Publication of the article:
«Bulletin of problems biology and medicine», 2024 Issue 2, 173, 400-403 pages, index UDC 611.013/.81/.82
