Ivakhniuk T. V., Smiianov V. A., Smiianova O. I., Shtainberher R. M., Pikhtirova A. V., Makarenko O. M., Holubnycha V. M.

MICROBIAL SPECTRUM AND ANTIMICROBIAL SUSCEPTIBILITY OF OTOPATHOGENS ISOLATED FROM PATIENTS WITH OTITIS

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Ivakhniuk T. V., Smiianov V. A., Smiianova O. I., Shtainberher R. M., Pikhtirova A. V., Makarenko O. M., Holubnycha V. M.

Heading:

MICROBIOLOGY

Type of article:

Scientific article

Annotation:

Otitis are common purulent-inflammatory diseases characterized by a diverse spectrum of microorganisms and varying susceptibility to antimicrobial agents. This study was conducted at the bacteriological laboratory of Sumy State University. The aim of the study was to determine the species composition of pathogens causing otitis media and otitis externa, as well as their antibiotic resistance. The study subjects were clinical isolates of microorganisms obtained from patients with otitis media and otitis externa. Methods: clinical, culture-based, and statistical. A high frequency (73.0% of cases) of polymicrobial associations was found in otitis: two-component microbial associations were detected in 41.3% of cases, three-component in 23.8%, and four-component in 7.9%. Typical otopathogens were Gram-positive cocci (59.7%), Gram-negative rods (27.6%), and yeast-like fungi of the genus Candida (12.7%). This study demonstrated variable levels of resistance of otopathogens to antimicrobial agents, which depended on the species composition of the microbiota. Among Gram-positive otopathogens, a high level of resistance to antimicrobial agents was detected in clinical isolates of S. aureus, which exhibited resistance to antibacterial agents of the macrolide group, aminoglycosides (amikacin), glycopeptides, third-generation cephalosporins, and lincosamides. Among Gram-positive otopathogens, high levels of antimicrobial resistance were detected in clinical isolates of S. aureus, which exhibited resistance to macrolides, aminoglycosides (amicacin), glycopeptides, third-generation cephalosporins, and lincosamides. Among Gramnegative otopathogens, a high level of multidrug resistance to antibacterial agents was detected in E. coli, K. pneumoniae, and P. aeruginosa. Clinical isolates of Candida spp., which were associated with S. aureus in 100% of cases, exhibited a high level of resistance to nystatin (82%) and miconazole (70.6%). The data obtained indicate the predominance of polymicrobial purulent-inflammatory processes in the context of otitis and variable resistance profiles of otopathogens, pointing to the need for etiologically oriented diagnosis and personalized therapy.

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Publication of the article:

«Bulletin of problems biology and medicine», 2026 Issue 2, 181, 237-242 pages, index UDC 579.222:616.28-002:615.281

DOI:

10.29254/2077-4214-2026-2-181-237-242