Duzhyi I. D., Myronov P. F., Ivakhniuk T. V., Pshenychnyi R. M., Holubnycha V. M.

Ag/Cu NANOCOMPOSITES AS AN ALTERNATIVE TREATMENT FOR INFECTIONS CAUSED BY MULTIDRUG-RESISTANT E. coli STRAINS

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About the author:

Duzhyi I. D., Myronov P. F., Ivakhniuk T. V., Pshenychnyi R. M., Holubnycha V. M.

Heading:

MICROBIOLOGY

Type of article:

Scientific article

Annotation:

The widespread spread of multidrug-resistant bacteria poses a serious threat to the healthcare system, dramatically reducing the effectiveness of most existing antibiotics. This situation significantly complicates the treatment of bacterial infections, prolongs the course of diseases, and increases the number of complications and mortality rates. One of the most promising areas is nanotechnology, particularly the synthesis and use of nanocomposites of metals such as silver and copper, which have a synergistic effect. Aim: to synthesize and study the antibacterial activity of Ag/Cu nanocomposites against a multidrug-resistant clinical strain of E. coli. The Ag/Cu nanocomposites were synthesized at the Department of Theoretical and Applied Chemistry of Sumy State University by the polyol-colloid method with low-frequency ultrasonic treatment. Broth serial dilutions determined the antibacterial activity of aqueous solutions of Ag/Cu nanocomposites according to the European Committee's recommendations for the Susceptibility Testing of Antibiotics (EUCAST). A clinical multidrug-resistant isolate of E. coli was used to determine the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of the Ag/Cu nanocomposites solution. The synthesized Ag/Cu nanocomposites were 45-100 nm in size, predominantly round in shape, and prone to agglomeration. The minimum inhibitory concentration of Ag/Cu nanocomposites for E. coli was 7.81 μg/ml, the minimum bactericidal concentration was 15.63 μg/ml, which is 16 times less compared to copper nanoparticles and 32 times less than silver nanoparticles. The disc diffusion method confirmed the bactericidal properties of multidrug-resistant E. coli. Changes in the morphological structure of E. coli were studied by transmission electron microscopy: membrane roughness, changes in cell shape, intracellular redistribution of cytoplasmic components/structures, cell wall damage, and partial leakage of cellular contents. The obtained results emphasize the potential of Ag/Cu nanocomposites as an effective antibacterial agent, particularly against multidrug-resistant strains of E. coli. This opens up prospects for their further use in medical practice, particularly for treating purulent inflammatory processes caused by multidrug-resistant bacteria.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 4, 175, 518-525 pages, index UDC 579.2:546.56/57:617-002.3

DOI:

10.29254/2077-4214-2024-4-175-518-525