Vygivska L. A., Nakonechna O. A., Pliekhova O. O.

GENERATION OF REACTIVE OXYGEN SPECIES IN ERYTHROCYTES AND ERYPTOSE PROCESSES IN ADOLESCENTS WITH ABNORMAL UTERINE BLEEDING

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About the author:

Vygivska L. A., Nakonechna O. A., Pliekhova O. O.

Heading:

CLINICAL AND EXPERIMENTAL MEDICINE

Type of article:

Scientific article

Annotation:

Abnormal uterine bleeding is one of the most common disorders of the pubertal period, affecting not only the reproductive function, but also the body as a whole. The study of the generation of reactive oxygen species in erythrocytes in adolescents with AUBA contributes to the understanding of the pathophysiological processes of its occurrence. Aim of the study: to determine the level of ROS generation in peripheral blood erythrocytes and eryptosis processes in patients with AUBA and anemia of varying degrees of severity. The study involved examination of 31 patients aged 10-17 years with AUB, which made up the main group (MG). Depending on the severity of anemia, the girls of MG were divided into subgroups: I – girls with AUBA and mild anemia, II – girls with AUBA and moderate anemia, III – girls with AUBA and severe anemia. Control group (CG) included 12 practically healthy girls. The level of ROS generation in peripheral blood erythrocytes was determined using flow cytometry. The mean fluorescence intensity of dichlorofluorescein in the population of viable peripheral blood erythrocytes was determined. In order to determine the binding of annexin V to phosphatidylserine to study the intensity of eryptosis processes, the following were analyzed: the percentage of annexin V – positive erythrocytes and the value of the mean fluorescence intensity of annexin V -FITC in all erythrocytes. In groups II and III, the level of generation of ROS in erythrocytes exceeded by 159.4%, 185.3% and by 78.8%, 96.6%, respectively, the level of this indicator in the CG and in Group I. The mean fluorescence intensity of annexin V-FITC in erythrocytes in patients of Group II and III was increased by 296.5% and 380.5%, respectively, compared to CG. Increased generation of reactive oxygen species in erythrocytes of peripheral blood is observed in patients with AUBA and with grades 2 and 3 anemia, which contributes to the development of oxidative stress and leads to an increase in the intensity of the processes of eryptosis of circulating erythrocytes.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 4, 175, 275-282 pages, index UDC 618.14-005.1-008.851-078-053.6

DOI:

10.29254/2077-4214-2024-4-175-275-282