Bakun O. V.

DIAGNOSIS OF ENDOMETRIOSIS BY METHODS OF DIFFUSE INTROSCOPY OF HISTOLOGICAL SECTIONS OF ENDOMETRIUM BIOPSY

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About the author:

Bakun O. V.

Heading:

CLINICAL AND EXPERIMENTAL MEDICINE

Type of article:

Scientific article

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The relationship between the optical (anisotropy of biological crystals) and geometric (hierarchical structure of birefringent fibrils) structure of the extracellular matrix of histological sections of biological tissues of various types with the ranges of changes in half-width values and the dispersion of fluctuations of the autocorrelation function of the coordinate distributions of the Jones matrix elements was experimentally revealed and theoretically substantiated. It was established that the physical reason for the decrease in the half-width of the correlation function and the increase in the dispersion of its fluctuations is the increase in the dispersion of orientations and birefringence of biological crystals. This article contains the results of endometriosis diagnosis research of histological sections of endometrial biopsy using the methods of diffuse Muller-matrix endoscopy. The aim of the study – to assess the effectiveness of endometriosis diagnosis using the methods of diffuse Muller-matrix introscopy of histological sections of endometrial biopsy. The study included 127 patients with infertility, who were divided into groups as follows: 59 women with endometriosis associated with infertility (experimental group) and 68 women with tuboperitoneal infertility (control group). Two groups of samples were studied: a biopsy of “healthy” uterine tissue obtained during diagnostic hysteroscopy, a control group 1: a biopsy of endometrial tissue with endometriosis, experimental group 2. Histological sections were made according to the standard technique on a microtome with quick freezing. A high level of parameters of the diagnostic power of detecting endometriosis was revealed by using a set of statistical markers of the method of polarization-phase Müller-matrix introscopy of linear birefringence thesiograms of native histological sections of endometrial biopsy: 1) very good diagnostic accuracy using statistical markers of the 1st and 2nd orders (SM1;SM2 – 92,1% – 93,7%); 2) excellent diagnostic accuracy using statistical markers of the 3rd and 4th orders (SM3;SM4 – 97,1% – 98,4%). Diagnostic markers of this pathological process are a statistically significant increase (pi=1;2;3;4<0,05) of the average value and variance of theziograms of coordinate distributions of random values of circular birefringence fluctuations. The values of asymmetry and kurtosis, on the contrary, decrease. Therefore, the application of the method of polarization-phase Muller-matrix introscopy of linear birefringence of fibrillar protein nets revealed an excellent level of diagnosis of endometriosis.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 4, 175, 252-265 pages, index UDC 618.145-007.415-076

DOI:

10.29254/2077-4214-2024-4-175-252-265