Feskov O. M., Zhylkova Ye. S., Bezpechna I. M., Yehunkova O. V., Blazhko O. V., Chumakova N. O., Feskova A. O.

RESULTS OF PREIMPLANTATION GENETIC TESTING OF BLASTOCYSTS OBTAINED FROM PATIENTS WITH SEVERE FERTILITY FAILURES

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Feskov O. M., Zhylkova Ye. S., Bezpechna I. M., Yehunkova O. V., Blazhko O. V., Chumakova N. O., Feskova A. O.

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BIOLOGY

Type of article:

Scientific article

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According to the literature, the male factor is the cause of infertility in almost 60% of cases. Currently, research data on the impact of male fertility disorders on embryo development and the occurrence of chromosomal abnormalities are ambiguous. The aim of this study was to investigate the dependence of the frequency of formation and the presence of changes in the karyotype of blastocysts when embryos are obtained by in vitro fertilization from men with severe spermatogenesis disorders on such indicators as concentration, motility, and morphology of spermatozoa in the ejaculate. In total, preimplantation genetic testing for aneuploidy (PGT-A) was performed on 974 blastocysts obtained from patients with spermatogenesis disorders and men with normal fertility. Microscopic analysis of the ejaculate was performed according to the WHO guidelines of 2010. Embryos were obtained using assisted reproductive technologies. Preimplantation genetic screening of embryos at the blastocyst stage was performed using a new-generation sequencing method. Statistical hypotheses were tested at the significance levels of 0.05, 0.025, and 0.01. The proportion of euploid embryos obtained from patients with severe spermatogenesis disorders is statistically significantly lower than that of blastocysts with a balanced karyotype obtained from men with normal reproductive function (p<0.05). A direct correlation was found between the morphology, concentration and motility of spermatozoa in the ejaculate and the total frequency of blastocyst formation, regardless of the embryo karyotype (t=6.555, t=8.449 and t=3.354, respectively). The results obtained in this study indicate that the detection of significant abnormalities of spermogram parameters in the microscopic analysis of the ejaculate is an indication for preimplantation genetic screening for aneuploidy in embryos obtained from patients with spermatogenesis disorders.

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 4, 175, 201-208 pages, index UDC 618.111-008:612.621.31:575.113

DOI:

10.29254/2077-4214-2024-4-175-201-208