Shevchuk M. М., Volos L. I.

MORPHOMETRIC ANALYSIS OF THE LIVER MICROCIRCULATORY VESSELS IN WHITE RATS AFTER SIX WEEKS OF EXPERIMENTAL ADMINISTRATION OF HEMP SEED OIL

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About the author:

Shevchuk M. М., Volos L. I.

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PROSPECTS FOR THE DEVELOPMENT OF MORPHOLOGICAL AND CLINICAL RESEARCH

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Scientific article

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Hemp seed oil is obtained by cold pressing, which preserves all its nutritional value and contains essential polyunsaturated fatty acids omega 6 and omega 3 in an ideal ratio of 3:1, which is crucial for maintaining health and preventing chronic diseases. The aim of the study was to determine the morphometric parameters of the microcirculatory bed of the liver in rats after 6 weeks of experimental application of hemp seed oil. An experimental study was conducted on 26 sexually mature male white rats in compliance with bioethical standards over a period of 6 weeks. In the experimental group (14 rats), the single daily dose of hemp seed oil was 0.5 ml/kg, and in the control group (6 rats) – 0.1 ml/kg. The intact group consisted of 6 animals. At the end of the experiment, histological and morphometric studies of the microcirculatory vessels of the liver were performed. The difference was considered statistically significant at a minimum significance level of p<0.05. After 6 weeks of experimental use of hemp seed oil at a dose of 0.5 ml/kg/day, the correct organization of the microcirculatory vessels was established, the absence of dilation and engorgement of the central veins, sinusoids and interlobular arteries, which indicates the absence of congestive phenomena. Comparative morphometric analysis of the vessels of the liver microcirculatory bed after 6 weeks of experimental use of hemp seed oil demonstrated the absence of statistically significant changes in the average diameters of the vessels of the microcirculatory bed, in particular the vessels of the hepatic lobule triads, central veins, sublobular and collecting veins and sinusoids (p>0.05) with the control group and the group of intact animals. The results obtained indicate the safety of using hemp seed oil as a dietary supplement at a dose of 0.5 ml/kg/day.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2025 Issue 4, 179, addition, 82-85 pages, index UDC 616.36–018.2:005:582.635.38]-092.9

DOI:

10.29254/2523-4110-2025-4-179/addition-82-85