Zykova N. P., Nebesna Z. M., Kramar S. B., Yakubyshyna L. V.

MORPHOLOGY

Scentific article

Abstract. Functional activity of practically all organs and organ systems in human body is affected at thermal trauma, in particular a pancreas. Therefore, in recent years in the treatment of burns are widely used tools that reduce the negative effects of toxins on the affected organism, in particular, the crushed substrate of lyophilized xenoskin. Therefore, the aim of the work was to establish microscopic changes of the pancreas exocrine part in the dynamics after experimental thermal trauma under the conditions of using the crushed substrate of lyophilized xenoskin. Grade III burn (20% of body surface) was applied with copper plates at temperature 97-100 ºC on the epilated back skin surface of 40 experimental mature white rats for 10 seconds under thiopental sodium anesthesia. 1 day after the burn, an early necrectomy of the damaged areas of the skin was performed. Then cleared areas were covered with a lyophilized xenoskin crushed substrate. Decapitation of animals was performed on 7, 14, 21 days of the experiment. The preparation of histological speciments was carried out according to conventional methods. Microscopic examinations of the pancreas exocrine part on the 7th day of the experiment, found that the lobular structure of the organ was preserved, however, there was swelling of the connective tissue stroma. The vast majority of exocrine pancreatocytes were hypertrophied. The nuclei were enlarged, their nucleoli were cleary visible. An increasing number of zymogen granules in the apical zone of cells caused the increasing of their secretory activity. Amoung unchanged exocrinocytes, destructively altered cells were found singly in the lobes of the gland. The vascular wall is characterized by moderate edema of adventitia, destruction of myocytes in tunica media and a decreasing leukocyte infiltration. The stasis of red blood cells in some hemocapillaries was detected. In the late stages of the experiment (14-21 days) there was a gradual normalization of the pancreas structural components. Conical pancreatocytes with enlarged round euchromatin nuclei and clear visible nucleoli were observed in the basal part and in the supranuclear part of the cell a large number of zymogen granules. The vessels were characterized by moderate blood supply, decreased wall edema, destruction of smooth arterial myocytes. Single leukocytes were detected in their adventitia. The veins and venules remained blood-filled. The vessels of the microcirculatory bed are moderately blood-filled without signs of slagging. Thus, the use of lyophilized xenoskin crushed substrate after thermal injury activates intracellular regeneration, which contributes to the normalization of the pancreas structural components.

pancreas, thermal injury, xenoskin.

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«Bulletin of problems biology and medicine» Issue 1 (159), 2021 year, 209-212 pages, index UDK 611.37-091.8-02:616-001.17-085.361:599.731.1-035.51]-092.9

10.29254/2077-4214-2021-1-159-209-212