Hnatyuk M. S., Cholach S. Yu., Tatarchuk L. V., Stets N. Ya.
HISTOSTEREOMETRIC CHANGES IN THE VENTRICLES OF THE HEART OF WHITE RATS AT COBALT INTOXICATION
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About the author:
Hnatyuk M. S., Cholach S. Yu., Tatarchuk L. V., Stets N. Ya.
Heading:
MORPHOLOGY
Type of article:
Scientific article
Annotation:
Pathological damage to the heart and blood vessels is often detected in the clinic, and its diagnosis, treatment, and prevention are important medical and social problems today. Morphologists and clinicians are interested in elucidating the peculiarities of structural and functional reorganization of the structures and vascular bed of the heart chambers under the influence of chemical factors. The remodelling of myocardial parts during prolonged exposure of humans and animals to cobalt, which is a heavy metal, has not been thoroughly investigated. The structures of the heart ventricles of 62 sexually mature white male rats were studied morphologically and histostereometrically. They were divided into two groups. The first group consisted of 30 intact experimental animals, and the second group consisted of 32 rats injected daily into the stomach with cobalt chloride at a dose of 4 mg/kg for 30 days. The animals were euthanized by bleeding under thiopental anaesthesia. Histological microdissections were made from the ventricles of the heart to determine the area of cardiomyocytes, their nuclei, nuclear-cytoplasmic index, volumes of cardiomyocytes, connective tissue structures, microvessels, damaged cardiomyocytes, stromal-cardiomyocyte and capillary-cardiomyocyte ratios. Morphometric parameters were statistically analyzed. It was found that the area of cardiomyocytes in the left ventricle of white rats under the influence of cobalt chloride statistically significantly increased by 1.79 times, nuclei by 41.9%, nuclear-cytoplasmic index decreased by 20.3% (p<0.001). In the right ventricle, the above quantitative morphological parameters changed by 32.8%, nuclei – by 21.0%, and nuclear-cytoplasmic index – by 8.4% (p<0.001). The volume of damaged cardiomyocytes in the left ventricle of experimental animals with prolonged intoxication with cobalt chloride increased by 13.2 times with a statistically significant difference, and in the right ventricle by 7.3 times (p<0.001). Stromal-cardiomyocyte, capillary-cardiomyocyte, and nuclear-cytoplasmic rations were significantly altered in the heart ventricles under the conditions of modelled pathology, indicating marked disturbances in tissue and cellular structural homeostasis.
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Bibliography:
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Publication of the article:
«Bulletin of problems biology and medicine», 2024 Issue 4, 175, 538-544 pages, index UDC 616.36-089.87-06.616-91]-092.9
