Paidarkina A. P., Kushch O. G.

FEATURES OF THE DISTRIBUTION OF COLLAGEN FIBERS OF THE I-TH AND III-TH TYPES IN THE MESENTERY OF RATS DURING THE FORMATION OF THE ADHESION PROCESS

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About the author:

Paidarkina A. P., Kushch O. G.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

To date, the composites of the small intestinal mesentery adhesion, intercellular interactions, namely lymphocyte-fibroblastic contacts, and patterns of changes in the synthesis of collagen fibres in its composition in the modelling of the adhesive process in comparison with the norm remain not fully understood. The question of the morphology and topography of collagen fibres of the first and third types in the mesentery of the small intestine and their number in the modelling of the adhesion process remains open. The study aimed to identify collagen fibres of the I and III types in the adhesion of the small intestine mesentery, as well as to study their topography and functional activity in the normal state and progressive adhesion. Object and Methods: dissection, macroscopic, microscopic, histological (making film preparations), silver impregnation by Laidlaw method, computer microdensitometry, mathematical (morphometric grid - the number of immunocompetent cells per standard area of 1000 μm2 was counted), statistical by Student. On the 7th, 14th and 21st day of modelling the adhesion process in rats in the mesentery of the small intestine, an increase in the intensity of collagen formation processes is observed, as indicated by a significant thickening of collagen fibres of the first and third types, as well as an increase in their area by 10,000 μm2. In the experimental group, on day 21, the relative area of type III collagen fibres increased to 41.074±0.07%, and type I collagen to 26.123±0.24% per standard unit of small intestinal mesentery area compared to the intact group (type I collagen - 1.086±0.03%, and type III collagen - 0.74±0.11% per standard unit of small intestinal mesentery area). Against the background of an increase in the number of lymphocytes per standard unit area of the small intestinal mesentery with progressive processes of adhesion formation, there is an increase in the protein-synthesising function of fibroblasts, which indicates the morphogenetic function of lymphocytes.

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Bibliography:

1. Fatehi Hassanabad A, Zarzycki AN, Jeon K, Deniset JF, Fedak PWM. Post-Operative Adhesions: A Comprehensive Review of Mechanisms. Biomedicines. 2021;9(8):867.
2. Shenkin A, Fraser WD, Series J, Winstanley FP, McCartney AC, Burns HJ, et al. The serum interleukin 6 response to elective surgery. Lymphokine Res. 1989;8:123–127.
3. Fischer A, Koopmans T, Ramesh P, Christ S, Strunz M, Wannemacher J, et al. Post-surgical adhesions are triggered by calcium-dependent membrane bridges between mesothelial surfaces. Nat. Commun. 2020;11:3068.
4. Foster DS, Marshall CD, Gulati GS, Chinta MS, Nguyen A, Salhotra A, et al. Elucidating the fundamental fibrotic processes driving abdominal adhesion formation. Nat. Commun. 2020;11:4061.
5. Wang Q, Huang Y, Zhou R, Wu K, Li W, Shi L, et al. Regulation and function of IL-22 in peritoneal adhesion formation after abdominal surgery. Wound Repair Regen. 2020;28:105–117.
6. Volyansʹka AG, Syvokonyuk OV. Porivnyalʹnyy analiz vplyvu sulʹfatubariyu ta talʹku na intensyvnistʹ spaykovoho protsesu u samostiynykh bilykh shchuriv. Intehratyvna antropolohiya. 2012;1(19):58-61. [in Ukrainian].
7. Bénézech C, Jackson-Jones LH. ILC2 Orchestration of Local Immune Function in Adipose Tissue. Front. Immunol. 2019;10:171-176.
8. Paidarkina AP, Kushch OG. Vyvchennya morfolohichnykh osoblyvostey ocherevyny bilykh shchuriv ta metodu yiyi zaboru. Morfolohiya. 2023;17(3):163-168. [in Ukrainian]
9. Terri M, Trionfetti F, Montaldо С, Cordani М. Mechanisms of Peritoneal Fibrosis: Focus on Immune Cells–Peritoneal Stroma Interactions. Frontiers in Immunology. 2021;12:1-17.
10. Voloshyn MA. Limfotsyt - faktor morfohenezu orhaniv. Zbirnyk tez dopovidey naukovo-praktychnoyi konf. Morfolohichni doslidzhennya - vyklyky suchasnosti; 2015 Kvit 23-24; Kyiv. Sumy: SumDU; 2015. s. 5-6. [in Ukrainian].
11. Soleimani A, Asgharzadeh F, Rahmani F, Avan A, Mehraban S, Fakhraei M, et al. Novel oral transforming growth factor-β signaling inhibitor potently inhibits postsurgical adhesion band formation. J. Cell. Physiol. 2020;235:1349–1357.
12. Paidarkina AP, Kushch OG. Peritoneal remodeling and changes in its lymphoid component in experimental modeling of adhesion disease in rats. ScienceRise: Biological Science. 2024;1(38):10–16. [in Ukrainian].
13. Kushch OG, Zlobina OV. Osoblyvosti budovy platsenty i limfoyidnoyi tkanyny, asotsiyovanoyi z platsentoyu u II-u periodi khvoroby u shchuriv. Sb. tezysov I mezhdunar. internet-konferentsiyi molodykh uchenykh i stud Sovremennye dostyzhenyya medytsynskoy y farmatsevtycheskoy nauky; 2012 Zhovt 23-25; Zaporizhzhya. Zaporizhzhya: ZHMU; 2012. s. 15-16. [in Ukrainian]
14. Kushch OG, Voloshyn MA. The relationship between the morphofunctional state of the lymphoid tissue of the skin of a newborn and the placenta. SMB. 2009;3-2:92-95.
15. Thaler MD, Judith A, Rong HZ, Mariana MD, Gary RІ, Matthew SY, et al. Expression of connective tissue growth factor in intra-abdominal adhesions. Dis Colon Rectum. 2002;45(11):1510-1519.

Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 2, 173, 391-399 pages, index UDC 57.085:[599.323.452:591.438]

DOI:

10.29254/2077-4214-2024-2-173-391-399