Aksamytieva M. V.

MORPHOLOGY

Scentific article

. To date, the blood vessels in allergic diseases of the respiratory system are an acting problem. It is known that the full functioning of tissues and organs depends on the state of vessels of a hemomicrocirculatory bed. The purpose of the work is to study morphological changes in the vessels of the hemomicrocirculatory channel of the guinea pig trachea with experimental ovalbumin-induced allergic inflammation. Material and methods. With the help of histological, morphometric and statistical methods studied the trachea of 48 male guinea pigs in conditions of experimental ovalbumin-induced allergic inflammation, which was modeled by subcutaneous sensitization and the subsequent inhalation of ovalbumin. To determine the structural and functional adjustment of arterioles, venules and blood capillaries, the outer diameter, diameter of the lumen, the thickness of the middle membrane and the cereal index of arterioles, the diameter of the lumen of venules and capillaries were determined. The results. It has been found that in all study groups, the size of the outer diameter of arterioles is increased (P */** <0.05), the maximum index is installed in the 3rd group, an increase of 2 times compared to the control group. Growth of the diameter of the lumen (P */** <0.05) found in all groups of research, maximum growth of 1.4 times installed on 36th day of the experiment (3rd group). Statistical enlargement (P */** <0.05) The middle membrane of the arteriol is detected in all experimental groups, increased increased in the late experiment. Growth of the index of the kernoghan index in 1st, 2nd, 3rd and 4th experimental groups (respectively 1.7; 2; 1.7 and 1.9 times) compared to the analogous control group (P */** <0.05). Increasing the diameter of the lumen of muscle venules was observed in all experimental groups, the maximum indicators were observed in the late experiment period. The most significant changes in the diameter of the lumen of blood capillaries of the trachea are almost 1.5 times less than a similar indicator of the control group recorded in the 4th group of research.

arteriol, venal, capillary, trachea, experimental allergic inflammation, ovalbumin, Guinea pig.

1. GINA. Global Strategy for Asthma Management and Prevention (GINA). Report 2018 [Internet]. 2018. Available from: http://ginasthma. org/2018-gina-reportglobal-strategy-forasthma-management-and-prevention/.
2. Denney L, Byrne AJ, Shea TJ, Buckley JS, Pease JE, Herledan GMF, et al. Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function. Immunity. 2015;43(5):945-58. DOI: https://dx.doi.org/10.1016%2Fj. immuni.2015.10.012.
3. Dotsenko SYa, Yatsenko OV. Elastic properties of pulmonary artery in chronic obstructive pulmonary disease. Zaporozhye medical journal. 2017;19(1):26-9. DOI: https://doi.org/10.14739/2310-1210.
4. Herasymiuk IE, Vatsyk MO. Osoblyvosti remodelyuvannya krovonosnykh sudyn lehenʹ shchuriv pry zastosuvanni riznykh metodiv rehidratatsiyi pislya zahalʹnoho znevodnennya. Visnyk problem biolohiyi i medytsyny. 2019;1(2):272. DOI: https://doi.org/10.29254/2077-4214- 2019-1-2-149-272-276. [in Ukrainian].
5. Hnatjuk MS, Tatarchuk LV. Morphometric analysis remodeling vessels hemomicrocirculatory bed of jejunum at resections of liver. Reports of Morphology. 2018 Mar 29;24(1):16-20. DOI: https://doi.org/10.31393/ morphology-journal-2018-24(1)-03.
6. Pronina OM, Koptev MM, Bilash SM, Yeroshenko GA. Response of hemomicrocirculatory bed of internal organs on various external factors exposure based on the morphological research data. World of Medicine and Biology. 2018;14(63):153. DOI: https://doi.org/DOI 10.26.724 / 2079-8334-2018-1-63-153-157.
7. Ha EH, Choi J-P, Kwon H-S, Park HJ, Lah SJ, Moon K-A, et al. Endothelial Sox17 promotes allergic airway inflammation. Journal of Allergy and Clinical Immunology. 2019 Aug;144(2):561-573. DOI: https://doi.org/10. 1016/j.jaci.2019.02.034.
8. Nebesna ZM, Yeroshenko GA. Histolohichni ta histokhimichni zminy lehenʹ pry eksperymentalʹniy termichniy travmi. Svit medytsyny ta biolohiyi. 2015;2(49):141-145. DOI: https://womab.com.ua/ua/smb-2015-02-2/5084. [in Ukrainian].
9. Reichard A, Asosingh K. Endothelial Cells in Asthma [Internet]. London: IntechOpen; 2019 [cited 2022 Feb 2]. Available from: .
10. Zemmouri H, Sekiou O, Ammar S, El Feki A, Bouaziz M, Messarah M, et al. Urticadioica attenuates ovalbumin-induced inflammation and lipid peroxidation of lung tissues in rat asthma model. Pharmaceutical Biology. 2017;55(1):1561-8. DOI: https://doi.org/10.1080/1388020 9.2017.1310905.
11. Adner M, Canning BJ, Meurs H, Ford W, Ramos Ramírez P, van den Berg MPM, et al. Back to the future: re-establishing guinea pig in vivo asthma models. Clinical Science. 2020 Jun;134(11):1219-42. DOI: .
12. Lambrecht BN, Hammad H. The immunology of asthma. Nature Immunology [Internet]. 2014 Dec 18;16(1):45-56. Available from: . DOI: https://doi.org/10.1038/ni.3049.
13. Vasconcelos LHC, Silva M da CC, Costa AC, Oliveira GA, Souza ILL, Queiroga FR, et al. A Guinea Pig Model of Airway Smooth Muscle Hyperreactivity Induced by Chronic Allergic Lung Inflammation: Contribution of Epithelium and Oxidative Stress. Frontiers in Pharmacology. 2019 Jan 24;9:1547. DOI: https://doi.org/10.3389/fphar.2018.01547.
14. Aksamytieva MV, Popko SS, Evtushenko VM. Morfolohichni zminy obolonok trakheyi morsʹkykh svynok pry eksperymentalʹnomu ovalʹbuminindukovanomu alerhichnomu zapalenni dykhalʹnykh shlyakhiv. Morphologia. 2021;15(1):22-7. DOI: https://doi.org/10.26641/1997-9665. 2021.1.22-27. [in Ukrainian].
15. Singh B, Shinagawa K, Taube C, Gelfand EW, Pabst R. Strain-specific differences in perivascular inflammation in lungs in two murine models of allergic airway inflammation. Clinical and Experimental Immunology. 2005 Aug;141(2):223-9. DOI: https://doi.org/10.1111/j.1365- 2249.2005.02841.x.
16. Antwi AO, Obiri DD, Osafo N. Stigmas-terol modulates allergic airway inflammation in guinea pig model of ovalbumin-induced asthma. Mediators Inflamm. 2017;2017:2953930. DOI: https://doi.org/10.1155/2017/2953930.
17. Popko SS. Morfolohichni zminy sudyn yemnisnoyi lanky hemomikrotsyrkulyatornoho rusla lehenʹ morsʹkykh svynok, sensybilizovanykh ovalʹbuminom. Zdobutky klinichnoyi i eksperymentalʹnoyi medytsyny. 2021;1(1):111-8. DOI: https://doi.org/10. 11603/1811- 2471.2021.v.i1.11691.
18. Cai Z, Liu J, Bian H, Cai J. Albiflorin alleviates ovalbumin (OVA)-induced pulmonary inflamma-tion in asthmatic mice. Am. J. Transl. Res. 2019;11(12):7300-7309. DOI: https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC6943473.

«Bulletin of problems biology and medicine» Issue 1 (163), 2022 year, 248-253 pages, index UDK 611.16:611.23].018:616-021.5-092.9].08:599.324.7

10.29254/2077-4214-2022-1-163-248-253