The aim of the study. The study of the impact of the mixture of alcohols (40 % ethanol and 100 % methanol) at a ratio of 3:1 in dynamics on the ultrastructure of the vascular and retinal shells of rats. into 3 groups: 1st group (experimental) – intraperitoneal single injection (ISI) to rats of a mixture of alcohols (40 % ethanol and 100 % methanol); 2nd group (experimental) – ISI of 100 % methanol. The methanol dose in the two study groups was 2.5 g/kg of rats’ body weight, 3rd group (control) – the ISI rats’ injection in a similar dose. For the rats, the effect of LD50 with ISI of methanol is 9.5 g/kg of body weight. Injections and euthanasia were administered in accordance with the requirements of the European Convention (Strasbourg, 1986). With help of electron-microscop had been studied the endothelial cells of vessels and capillaries of the choroid, pigmented epithelium of the retinal, its photoreceptor and ganglion cells, as well as processes of the Muller’s rat’s cells in 1 and 3 hours, 1, 3, and 7 days after ISI in a transmission electron microscope TEM-100-01. Results and discussion. 1 hour after the injection of a mixture of the alcohols endothelial cells (EC) vessels were in a state of edema, and in choriocapillaris (CC) only a single EC. The lumen of XO vessels and capillaries, as well as the main substance of the Bruch’s membrane, had high electron density. Fenestra EC CC were virtually absent. In the layer of the retinal pigment epithelium (RPE) were damaged organelles of cells of varying degrees of severity, until their complete destruction with focal destruction of the plasmalemma on the basal and apical sides of the cell. These cells also showed an increase in the number of lysosomes. Phagosomes were practically absent in them, indicating that the process of phagocytosis of the disks of the outer segments of photoreceptor cells was impaired. Other layers of the retina were characterized by slight hydropic changes. In the dynamics (up to 7 days) in all the investigated structures of the vascular and retinal shells revealed hydropic degeneration progressed. These structures, especially in RPE cells, also showed signs of a compensatoryregenerative behavior, aimed at enhancing energy-forming function and white-synthetic processes. After methanol injection, the pathological changes in these tissues were the same as those after the injection of the alcohol mixture, but they were more profound. Conclusions 1. A mixture of alcohols in which the methanol dose was 2.5 g/kg body weight of the rat causes significant ultrastructural changes in the endothelial cells of the choroid vessels and in the cells of the rat retinal pigment epithelium within 1 hour after its introduction. In the period from 3 hours to 7 days of observation, the destructive processes in the structures of the vascular and retinal membranes progress with the parallel manifestation of compensatoryrestorative processes in them. 2. 100% methanol at the above dose causes the same type of changes in the investigated elements of the vascular and mesh, as the mixture of alcohols, but they are more profound.
ultrastructure, vascular, capillary, choroid, retinal pigment epithelium, retina’s photoreceptor cells, retina’s ganglion’s cells, processes of the Muller’s cells.
«Bulletin of problems biology and medicine» Issue 4 Part 1 (153), 2019 year, 224-227 pages, index UDK 617.723/.35-005:616.547.26-778.317-092.9
