Fik V. B., Fedechko J. M., Paltov Ye. V., Kryvko Yu. Ya.
The microbial spectrum of the oral cavity of opioid-dependent individuals is characterized by qualitative diversity and quantitative characteristics. In order to successfully treatment purulent-inflammatory processes, it is necessary to determine as soon as possible not only the composition of the microflora, but also to determine the sensitivity of antibiotics. Methods. In order to select an antibacterial drug for the correction of dysbiotic changes that develop in the cervical part of the teeth and gingival margin under the action of opioids, the antibiotic sensitivity of bacterial isolates was determined by disco-diffusion method to ciprofloxacin, cefazolin and ceftriaxone. Isolates of pathogenic and conditionally pathogenic bacterial species from the studied subbiotope of the oral cavity were isolated at the end of the sixth and tenth weeks of opioid exposure, in which animals were administered the opioid analgesic nalbuphine in medium therapeutic doses. Results. After six weeks of nalbuphine’s exposure, 85.7% of Staphylococcus aureus, 85.3% of Staphylococcus intermedius, 83.3% of coagulase-negative staphylococci, 87.5% of Streptococcus pyogenes, 76.9% of Escherichia coli, 76% hemolytic Escherichia coli were highly sensitive to ciprofloxacin. 82.1% of Staphylococcus aureus, 82.4% of Staphylococcus intermedius, 80.0% of coagulase-negative staphylococci, 81.2% of Streptococcus pyogenes, 73.1% of Escherichia coli and 72.0% of hemolytic Escherichia coli were highly sensitive and sensitive to cefazolin. The highest activity against these species of bacteria was detected to ceftriaxone, in particular, highly sensitive and sensitive to this antibiotic were 92.9% Staphylococcus aureus, 91.2% Staphylococcus intermedius, 86.7% coagulasenegative staphylococci, 90.6% Streptococcus pyogenes 80,8% Escherichia coli, 80.0% hemolytic Escherichia coli. After ten weeks of opioid action, it was noted that 82.1% of Staphylococcus aureus, 82.4% of Staphylococcus intermedius, 83.3% of coagulase-negative staphylococci, 84.4% of Streptococcus pyogenes, and 76.9% of Escherichia coli, 72.0% of hemolytic Escherichia coli the intestine were highly sensitive to ciprofloxacin. A highly sensitive and sensitive degree to cefazolin was noted – 78.6% Staphylococcus aureus, 79.4% Staphylococcus intermedius, 76.7% coagulase-negative staphylococci, 78.1% Streptococcus pyogenes, 69.2% Escherichia coli, 68.0% hemolytic Escherichia coli. 89.3% of Staphylococcus aureus, 88.2% of Staphylococcus intermedius, 86.7% of coagulase-negative staphylococci, 87.5% of Streptococcus pyogenes, 80.8% of Escherichia coli and 76.0% of hemolytic Escherichia coli were highly sensitive and sensitive to ceftriaxone. Conclusion. Determining the antibiotic susceptibility of microorganisms isolated in the cervical part of the teeth and gingival margin of animals exposed to long-term opioid, it was found that isolates of bacteria – potential pathogens of purulent-inflammatory process in the studied biotope were more sensitive and sensitive to ceftriaxone which is advisable to use in therapy for disorders of the microbiocenoses of the oral cavity under the action of the opioid analgesic nalbuphine.
antibiotic sensitivity, opioid analgesic, bacterial isolates, oral cavity, rats.
«Bulletin of problems biology and medicine» Issue 3 (157), 2020 year, 213-216 pages, index UDK 16.311.2-008.87-085.015.8:615.212.7
