Yuryk Ya. I.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Compression stress under crush syndrome leads to the activation of lipid peroxidation, which causes the development of endogenous intoxication. In response, the antioxidant defence system is activated. The aim of the study was to elucidate the features of antioxidant protection in heart and blood tissue under conditions of endogenous intoxication in the postcompression period of crush syndrome. Object and methods of research. The study was performed on 60 white laboratory rats. The experimental group consisted of 48 animals, which were divided into four subgroups of 12 individuals each, which were removed from the experiment 24 hours, 3, 7 and 14 days after simulation of crush syndrome. The control group consisted of 12 intact rats. Crush syndrome was simulated by compressing the soft tissues of the right pelvic limb under conditions of anesthesiain by intraperitoneal injection of ketamine hydrochloride (100 mg/kg body weight) in a specially designed device, the compression force was 7 kg/cm2 per 5 cm2 for 6 hours without damage main vessels and bones. The activity of serum catalase and heart homogenate was studied spectrophotometrically. Superoxide dismutase activity was determined in the heart homogenate. The concentration of ceruloplasmin was determined in the blood serum. The reliability of the obtained digital data was evaluated using the non-parametric Kruskal-Wallis test. Results. Compression stress under the conditions of crush syndrome causes the activation of the antioxidant defence system, which is manifested by the maximum increase in catalase activity, superoxide dismutase and ceruloplasmin concentration in the early postcompression period of crush syndrome. The activity of superoxide dismutase in heart tissue after 3 days of the research increased by 58%, and the concentration of ceruloplasmin in serum – by 67% compared with similar indicators in intact rats. Comparing the activity of catalase in serum and homogenate of the heart in intact and experimental animals we found the maximum increase of the letter in serum after 3 days of research in 4,1 times, and in heart tissue this indicator increased after 1 day in 3,7 times, and after 3 days statistically insignificantly decreased by 2,3%, that is practically has not changed. Starting from the 7 th day of the experiment catalase activity, superoxide dismutase and ceruloplasmin concentration decreased unidirectionally.

antioxidant protection, crush syndrome, blood, heart.

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«Bulletin of problems biology and medicine» Issue 4 (162), 2021 year, 214-217 pages, index UDK 612.01511-02:[611.12-018.61-611-018.5]-06:616.748.2-001.35-092.9

10.29254/2077-4214-2021-4-162-214-217