Volotovska N. V.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Under various environmental influences, the immune system responds sensitively. Implementation a protective function, however, it can also cause autoimmune processes, which will only aggravate the underlying disease, especially in conditions of already activated systemic response to inflammation. Thus, imbalance in the immune system can be one of the factors of the «vicious circle» that occurs on the background of a blood loss, when circulating immune complexes can contribute to hepato-renal syndrome. However, these concepts remain insufficiently studied in the conditions of combining massive blood loss with ischemia-reperfusion. The aim of the study – to know the dynamics of the concentration of circulating immune complexes, as well as the patterns of violations of humoral immunity due to the systemic effects of limb ischemia-reperfusion and massive blood loss. Object and methods of research. The work was performed on white nonlinear male rats, and the object of study was the course of massive blood loss under conditions of ischemia-reperfusion of the limb. In animals, limb ischemia-reperfusion was simulated by applying an elastic tourniquet to the upper third of the thigh according to the established method, massive blood loss from the femoral vein by femoral vein puncture was simulated, and in the third group these two interventions were combined. Results. The simulated interventions are characterized by pronounced disorders of humoral immunity, which are accompanied by accumulation in the serum of the CІC and the development of dizimunoglobulinemia. The content of Ig A in the conditions of blood loss combined with limb ischemia-reperfusion (EG-3) was characterized by two periods of growth on the 1 and 7 days after the intervention and was higher compared to other experimental groups. The content of Ig M in EG-3 increased on the 1 h after reperfusion (by 53.3% compared with the control), but later remained at the lowest level. The content of Ig G increased in all study groups mainly up to the 3 day with a subsequent decrease. In EG-3 the index on the 14 day became significantly lower than the control (p <0.05). Conclusions. At all stages of development of traumatic disease there is a significant increase in the titer of immunoglobulins A, M, G with an predominance in the first hour after reinfusion, as well as an increase on the 7 day of experiment, that confirms a strong antigenic load involving both primary and secondary immune response in pathogenesis of post-traumatic changes. The peculiarity was that the combination of limb ischemia-reperfusion with blood loss can inhibit the formation of immunoglobulins.

massive blood loss, mechanical trauma, ischemia-reperfusion, circulating immune complexes, immunoglobulins.

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«Bulletin of problems biology and medicine» Issue 4 (162), 2021 year, 109-114 pages, index UDK 616.718-001.5:616.137/.147-005/1:616.748-005.4]-089.814-092.19

10.29254/2077-4214-2021-4-162-109-114