Shamelashvili K. L., Chernilovskaya S. V., Kopatskaya M. V., Vyselko A. D., Lushnya L. M., Makarets M. F., Davydenko I. V.

BIOLOGY

Scentific article

. Cadmium is a toxic heavy metal that accumulates in some tissues and contributes to the development of oxidative stress, which is a source of serious pathological conditions in the body. It is important to look for substances that could reduce the content of cadmium in the organs of a living organism and suppress its negative effects. The aim of the study was to experimentally determine the modifying effect of zinc succinate on the embryotoxicity of cadmium chloride during chronic intragastric administration throughout pregnancy in rats. Determine the degree of accumulation of cadmium and zinc in the blood and liver of pregnant rats. To determine the effect of cadmium and zinc succinate salts on the activity of aspartate aminotransferase and alanine aminotransferase. Experimental studies were performed on female Wistar rats. For embryonic research, female rats with a date of pregnancy were obtained using the method of vaginal swabs. From the first day of pregnancy, the test substances were administered. To influence and determine the toxic effect of cadmium chloride, we daily administered to females per os solution of cadmium chloride (at a dose of 2.0 mg / kg) throughout pregnancy. On the 13th day of pregnancy, a surgical slaughter was performed. The embryotoxic effect of the studied substances was assessed by the following indicators: 1. Total embryonic mortality; 2. Preimplantation mortality; 3. Postimplantation mortality; 4. The number of fruits per 1 female in the group. Quantitative determination of cadmium and zinc was performed by atomic emission analysis on an EMAS-200 CCD spectrometer. The enzymatic activity of ACT and ALT was determined according to the method of determination of these enzymes by Reitman-Frenkel. Assessment of the reliability of statistical studies was performed using Student’s t-test. The zinc succinate complex has a pronounced modifying effect on embryotoxic parameters of cadmium chloride in the studied dose, which is manifested in an increase in the number of embryos, reduction of all types of embryonic mortality with combined administration of cadmium chloride and zinc succinate in experiments. The joint administration of zinc and cadmium helps to reduce the concentration of cadmium in the liver and blood of pregnant female rats. Isolated administration of cadmium chloride increases the activity of ALT and AST, which may be associated with impaired liver cell integrity. With the introduction of a complex of cadmium chloride and zinc succinate, there is a decrease in the activity of these enzymes almost to the control level.The obtained data indicate a modifying effect of zinc succinate on embryotoxic and hepatotoxic properties of cadmium chloride when combined in these doses in an experiment in rats.

cadmium chloride, zinc succinate, embryogenesis, embryotoxicity, embryonic mortality, liver, ALT, AST.

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«Bulletin of problems biology and medicine» Issue 4 (162), 2021 year, 87-92 pages, index UDK 599.323.4:591.436:591.3:546.48:616-092.9

10.29254/2077-4214-2021-4-162-87-92