At crush syndrome, endothelial cells are the first to respond to myoglobin, lysosomal and mitochondrial enzymes, histamine, serotonin, anaerobic glycolysis products, and free radicals, which enterthe blood stream in high concentrations. The aim of the study was to determine the degree of morphofunctional remodeling of endothelial cells in the postcompression period of crush syndrome. Object and methods of research. The study was performed on 40 white laboratory rats. The experimental group consisted of 32 animals, which were divided into foursubgroups of 8 individuals each, which were removed from the experiment 24 hours, 3, 7 and 14 days after simulation of crush syndrome. The control group consisted of 8 intact rats. Crush syndrome was simulated by compressing the soft tissues of the right pelvic limb under anesthesiain a specially designed device, the compression force was 7 kg/ cm2 for 6 hours. Determination of the number of circulating in the blood desquamated endothelial cells (DEC) was performed by the method of J. Hladovec (1978) in the modification of V.V. Sivak (2007). Histological preparation were drew with hematoxylin and eosin. Results. The number of desquamated endothelial cells (DEC) in intact rats was (3,63±0,40)×104 /l, 24 hours after the experiment, the number of desquamated endothelial cells doubled and was (7,13±0,25)×104 /l (p<0,01). Three days later, the amount of SEC was (7,75±0,52)×104 /l, which was 2, 13 times more in compared to intact animals. After seven days, the number of DEC population (6,25±0,48)×104 /l, which was 1,7 times higher at p<0,05. 14 days after crush syndrome simulation, the number of DEC of the population (5,38±0,40)×104 /l, which was 15 times (p<0,05) compared to intact animals. Morphological examination reveals areas of denudation of intimate vessels, which are aimed at the release of endothelial cells, which load different sizes and change shape, there are cells with swelling cytoplasms, phenomena karyopyknosis and karyolysis.
«Bulletin of problems biology and medicine» Issue 3 (161), 2021 year, 287-290 pages, index UDK 616.132.1/2-003.93-091.8-02:6.16-001.32]-092.9
