Basysta K. I., Rodinskiy A. G., Guz L. V.

PHYSIOLOGY

Scentific article

Abstract. The article presents the results of studying the level of anxiety in rats of different ages (middle-aged rats and old rats) with experimental hyperglycemia. The animals were kept in standard vivarium conditions in the amount of 90 individuals. Each age group of rats was divided into two groups: intact and experimental with simulated experimental hyperglycemia. Experimental hyperglycemia was modeled by intraperitoneal administration of aloxane monohydrate solution (120 mg/kg, “Sigma”). Blood glucose was determined using a Bionime portable glucometerfor 10 days. On day 10, those animalsthat had a blood glucose level higherthan 28 mmol/l were selected. The analysis of the level of anxiety was performed using the method of «Raised cruciform labyrinth». The time of the latency period, the time in the center, open and closed sleeves, the number of measures in the closed sleeve, the number of viewsin the open sleeve and the number of views down from the open sleeve were recorded. In both age groups, the indicators of approximate research activity differed significantly (p<0.05). The time spent in open arms in middle-aged rats with diabetes was less than in intact animals. The same tendency was observed in older rats. Closed-sleeved rats in middle-aged hyperglycemia were longer than rats without diabetes. This time was essential for middle-aged rats, because such behavior is not typical for this age. In older animals, this rate also increased for rats with hyperglycemia. This suggests that rats with diabetes are more pronounced anxiety, which is provoked by changes in the environment. They have no interest in learning any thing new and fear. Also, rats with diabetes have reduced approximate research activity, which was found in the study of the open sleeve. Thus, it was found that experimental hyperglycemia had significant effects in rats, which manifested a state of anxiety.

: experimental hyperglycemia, diabetes mellitus, alloxan-induced diabetes, anxiety, «raised cruciform labyrinth».

1. Kondratenko AP. Kognitivni i emotsiyni porushennya u patsientok z tsukrovim diabetom 2 tipu. Zbirnik materialiv naukovo-praktichnoyi konferentsiyi studentiv, molodih vchenih ta likariv. Kharkiv international annual scientific meeting (KhIASM) 2020; 2019 Trav 22; Kharkiv. Kyyiv, Kharkiv: Ministerstvo osviti i nauki Ukrayini, Harkivskiy natsionalniy medichniy universitet; 2019. s. 107–108. [in Ukrainian].
2. Dubinina II, Zhadnov VA, Yankina SV, Solov’eva AV. Simptomy depressii i trevogi u bol’nykh sakharnym diabetom 2 tipa i tserebrovaskulyarnoy bolezn’yu. Sakharnyy diabet. 2012;15(4):59-62. DOI: https://doi.org/10. 14341/2072-0351-5539. [in Russian].
3. Trofimova GI, Gorshkov IP, Volynkina AP. Psikhologicheskiye osobennosti bol’nykh sakharnym diabetom. Prikladnyye informatsionnyye aspekty meditsiny. 2014;17(1):188-192. [in Russian].
4. Tukalenko EV. Povedinkovi reaktsiyi schuriv vistar u viddalenomu periodi pislya vnutrishnoutrobnogo oprominennya. Naukovi pratsi Chornomorskogo derzhavnogo universitetu imeni Petra Mogili kompleksu “Kievo-Mogilyanska akademiya”. 2016;280(268):99-104. Dostupno: http://nbuv. gov.ua/UJRN/Npchdutb_2016_280_268_20. [in Ukrainian].
5. Tsemenko KV, Kireev IV, Koshoviy OM. Otsinka emotsiyno-povedinkovoyi reaktivnosti u schuriv pislya vvedennya kompleksu brusnitsi zvichaynoyi v kombinatsiyi z argininom. Ukrayinskiy biofarmatsevtichniy zhurnal. 2019;1:50-54. DOI: /10.24959/ubphj.19.210. [in Ukrainian].
6. Tolmachova KS, Kireev IV, Koshoviy OM, Tsemenko KV, Botsula IV. Vivchennya emotsiyno-povedinkovoyi reaktsiyi u schuriv pislya vvedennya novogalenovogo fitokompleksu pagoniv Ledum palustre. Klinichna farmatsiya. 2019;3(23):48-51. DOI: 10.24959/cphj.19.1499. [in Ukrainian].
7. Lakin GV. Biometriya. M.: Vyisshaya shkola; 1990. 352 s. [in Russian].
8. Bahtiyarova ShK, Kapyisheva UN, Ablayhanova NT, Baimbetova AK, Zhaksyimov BI, Korganbaeva AA, et al. Povedenie zhivotnyih v razlichnyih testah. Mezhdunarodnyiy zhurnal prikladnyih i fundamentalnyih issledovaniy. 2017;8(1):92-96. Dostupno: https://www.appliedresearch. ru/ru/article/view?id=11767. [in Russian].

«Bulletin of problems biology and medicine» Issue 2 (160), 2021 year, 336-338 pages, index UDK 616.153.455-008.61-092.9-07:159.97-053

10.29254/2077-4214-2021-2-160-336-338