Pelypenko O. V.

MORPHOLOGY

Scentific article

Abstract. Introduction. One of the modern hypotheses of the pathogenesis of osteoarthritis directly relates to circulatory disorders in joint-forming tissues with the subsequent development of dystrophic-degenerative changes. Due to the absence of vessels in cartilage tissue, the study of hemocirculatory processes in the subchondral bone and synovial membrane in normal and in conditions of pathological processes becomes especially relevant. The aim of the research. Comparative assessment of the state of the subchondral bone and synovial membrane microcirculation in the knee joints of rats in normal, in the conditions of experimental arthritis and after exposure to cryopreserved placenta. Material and methods. The experimental research was performed on 115 white rats. 3 control groups of 35 animals in each group were compared with the intact group (10 animals), which performed: 1) modeling of carginan-induced aseptic inflammation of the knee joint; 2) subcutaneous implantation of a fragment of the placenta; 3) subcutaneous implantation of a fragment of the placenta on the background of aseptic inflammation of the knee joint. Histological specimens were prepared according to general methods with hematoxylin-eosin and Van Gizon staining. Results. Filling of vessels of bone and, especially, synovial fabrics was characteristic of the first three days of experiment in all groups of research. The reaction of the microcirculatory bed in the group where aseptic inflammation was simulated differed in the change of this state by the stage of tissue ischemia. In the group of animals injected with cryopreserved placenta, on the contrary, for a long time there was the presence of sludge-phenomenon that indicated the physiology and reversibility of the process. The injection of cryopreserved placenta on the background of aseptic inflammation leveled ischemic phenomena, intensified the processes of angio- and osteogenesis. Conclusions. The microcirculatory system of the synovial and skeletal components of the joint is synchronized in response to the influence of pathological factors. The synovial membrane, as more sensitive, is previously involved in metabolic transformations. The bone component is relatively stable – it later undergoes destructive changes and is reorganized earlier. The injection of cryopreserved placenta can significantly reduce the negative impact of the inflammatory process in experimental osteoarthritis.

osteoarthritis, cryopreserved placenta, microcirculation.

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«Bulletin of problems biology and medicine» Issue 2 (160), 2021 year, 227-230 pages, index UDK 616.72-002.77.599.323.4

10.29254/2077-4214-2021-2-160-227-230