Likhachov V. K., ShymanskaYa. V., Dobrovolska L. M., Makarov O. G.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Abstract. Introduction. It is known that during the late phase of the second trimester and the early phase of the third trimester of normal pregnancy there is a shift towards M2 phenotype macrophages, which prevent repulsive effects on the foetus. Macrophage imbalance contributes to the development of serious obstetric pathology. The aim of the study is to investigate the changes in the balance of decidual macrophages of subpopulations M1 and M2 in the placentas of healthy women and patients, who had in vitro fertilization and preterm labours, and to detect the factors activating these macrophages. Materials and methods. The study group consisted of 37 women, who had IVF pregnancies, and demonstrated prognostic signs of high risk of preterm labour and those, who refused preventive treatment. The comparison group consisted of 20 women with physiological pregnancies. We investigated the content of pro- and anti-inflammatory cytokines and the activity of inducible NO-synthase and arginase in cervical mucus samples. For the purposes of immunohistochemical study, 10 placentas of the women from the study group, who had preterm labours, and 5 placentas from the healthy women, had labours in 32-34 weeks due to the monochorionic monoamniotic twins were examined. Results and discussion. The findings demonstrate an imbalance between pro- and anti-inflammatory cytokines in favour of pro-inflammatory that is causally related to the imbalance between the activity of inducible NO-synthase and arginase in favour of iNOS. Such changes in immune factors contribute to an abnormal increase in the content of decidual macrophages of M1 subpopulation in the placentas of women with IVF pregnancies. Conclusions. Significant accumulation of decidual macrophages of the M1 subpopulation, which produce increased amounts of reactive oxygen and nitric species in the placentas of women with IVF pregnancy in the second trimester probably, contributes to the manifestations of placental dysfunction with subsequent preterm birth.

in vitro fertilization, cytokines, inducible NO-synthase, arginase, decidual macrophages.

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«Bulletin of problems biology and medicine» Issue 2 (160), 2021 year, 105-109 pages, index UDK 618.345-018-06:[618.177-089.888.11+618.396]

10.29254/2077-4214-2021-2-160-105-109