KovchunV.Yu., Sikora V. Z.

MORPHOLOGY

Scentific article

Abstract. Water deficiency in the body is a physiological condition, the underestimation of which can have significant consequences for the health and performance of people. The human body uses water every day to carry out physiological processes, the restoration of water balance occurs due to the consumption of liquid in sufficient quantities, which is the main prerequisite for human health. Dehydration shock is a terminal state that leads to irreversible changes in the acid-base state, water-electrolyte balance and cardiovascular activity. Therefore, understanding of risk factors, physiological processes and pathogenetic links is relevant. The aim of the study was to learn the histomorphometric features of the pancreatic parenchyma under the influence of a sublethal degree of general, cellular and extracellular dehydration of the body. To achieve the goal, 40 male laboratory rats were used: the control (10 rats) and experimental groups (30 rats), the control group was on a regular drinking diet, the experimental groups were simulated sublethal degree of dehydration according to the models of A. D. Soboleva, V. Z. Sikora, and Ya. Ya. Bodnar. The study of morphological features was carried out on days 10, 30 and 90 of the study. The study of micropreparations was carried out using the digital system “SEO Scan Lab 2.0”. Morphometric measurements were processed using the statistical program IBM SPSS Statistic 21. It was found that the histostructure of the pancreas undergoes pronounced changes under conditions of exposure to the sublethal degree of various types of dehydration. On the 10th day of general dehydration, there was a significant increase in the areas of the islets of Langerhans and pancreatocytes (p<0.0001). On the 30th day, a significant increase in the areas of the islets of Langerhans, the nuclei of the pancreatocytes and an increase in the nuclear-cytoplasmic ratio are observed simultaneously with a significant decrease in the area of the pancreatocytes and their cytoplasm (p<0.0001). On the 90th day of the study, a significant decrease in the areas of pancreatocytes and their cytoplasm was revealed, with a simultaneous increase in the area of the nuclei and the nuclear-cytoplasmic ratio (p<0.0001).

dehydration, pancreas, islets of Langerhans.

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«Bulletin of problems biology and medicine» Issue 1 (159), 2021 year, 213-215 pages, index UDK 611.37.018:616.395-092.9(047.31)

10.29254/2077-4214-2021-1-159-213-215