Bigulyak G. T., Klishch I. M.

DENTISTRY

Scentific article

The purpose of the study was to investigate the effect of stem cells on the activity of proteolysis processes in rats with periodontitis, corrected by stem cells. The results of the study of the proteinase system/proteinase inhibitors in animals with acute experimental periodontitis, which was corrected with the help of human mesenchymal stem cells, were analyzed. Depending on the treatment, the animals were divided into several groups: 1st group – animals without pathology (intact); 2nd – animals with simulated acute periodontitis; 3rd – animals with periodontitis corrected by mesenchymal stem cells. The observation dates were set on the 1st, 7th, 14th, and 21st days after the animals were removed from the experiment. Indicators characterizing the activity of proteolysis processes were determined (by lysis of azoalbumin, azocasein, and azocol, using a set of “SimkoLtd” (Ukraine) reagents and proteolysis inhibitors (α2-MG, α1-IP), and the proteolysis index was also calculated. According to the results of the study, it was established that during the experimental modeling of acute periodontitis, there was a pronounced activation of proteolysis with a maximum on the 1st day of observation and a slight increase in the level of protease inhibitors. A less pronounced increase in inhibitory potential, along with a significant increase in proteolytic activity, was reflected in a significant increase in the value of the proteolysis index. The use of MSCs reduces the imbalance of the protease/antiprotease system, which is accompanied by a decrease in the proteolysis index, especially in the later periods after injury.

acute experimental periodontitis,correction,stem cells,proteolysis,proteases,protease inhibitors.

1. Thumbigere Math V, Michalowicz BS, Hodges JS, Tsai ML, Swenson KK, Rockwell L, et al. Periodontal disease as a risk factor for bisphosphonate related osteonecrosis of the jaw. Journal of periodontology. 2014 Feb;85(2):226-33.
2. Melnychuk GM, Politun AM, Kovalchuk LE, Erstenyuk GM. Alhorytm vynyknennya y rozvytku heneralizovanoho parodontytu ta parodontozu schema kompleksnoho likuvannya heneralizovanoho parodontytu. Sovremennaya stomatolohyya. 2013;1:35-40. [in Ukrainian].
3. Danylevskyy NF, Borysenko AV. Zabolevanyya parodonta. K.: Zdorovʺya; 2008. 464 s.
4. Veremeenko KN, Holoborodʹko OP, Kyzym AY. Proteolyz v norme y pry patolohyy. K. : Zdorovʺya; 1988. 200 s.
5. Kresyun VY, Sementsiv NH, Reheda MS. Osoblyvosti zrushenʹ stanu proteyinazno inhibitornoyi systemy za umov rozvytku eksperymentalʹnoho alerhichnoho alʹveolitu i shlyakhy yoho korektsiyi. Odes. med. zhurn. 2009;3(113):35-7.
6. Krynytsʹka IYA. Stan proteyinazo-inhibitornoyi systemy krovi ta bronkhoalʹveolyarnoho zmyvu u shchuriv z modelʹovanym hepatopulʹmonalʹnym syndromom. Zah. patolohiya ta patolohichna fiziolohiya. 2012;7(4):92-7. [in Ukrainian].
7. Shvetsʹ VI, Kisilyuk VL, Shkrobanetsʹ ID. Zminy tkanynnoho proteolizu pry intoksykatsiyi bilykh shchuriv malymy dozamy vazhkykh metaliv. Klinich. ta eksperym. patolohiya. 2009;8(4):87-9. [in Ukrainian].
8. Dedulʹ MY, Radetskaya LE, Kyrpychenok LN. Systema proteolyza v syvorotke krovy y perytonealʹnoy zhydkosty pry khyrurhycheskom lechenyy bolʹnykh éndometryozom. Novosty khyrurhyy. 2006;14(3):74-80.
9. Zolotukhyna EL. Stvolovyekletky y perspektyvy ykh prymynenyya v stomatolohyy y chelyustno-lytsevoy khyrurhyy. Molodyy vchenyy. 2014;6:145-7.
10. Malanchuk VA, Astakhova VS, Tsylenko OL. Osteohennye kletky – predshestvennyky kostnoho mozha cheloveka v rekonstruktyvnovosstanovytelʹnoy khyrurhyy. Zhurn. AMN Ukrayny. 2009;2:276-88.
11. Malanchuk VO. Rekonstruktyvno-vidnovleni operatsiyi na nyzhniy shchelepi (kliniko-laboratorne doslidzhennya) [avtoreferat]. Kyyiv; 1994. 47 s. [in Ukrainian].
12. Khisham A. Pulʹpit: osoblyvosti rozvytku ta vybir metodu likuvannya [avtoreferat]. Kyyiv; 1993. [in Ukrainian].
13. Saber SE. Tissue engineering in endodontics. Journal of Oral Science. 2009;51(4):495-507.
14. Tsilenko OL, Sosidko AV. Sravnitelʹnaya kharakteristika tipov koloniy KOYe – f pulʹpy zuba I stvolovykh stromalʹnykh kletok kostnogo mozga cheloveka v usloviyakh in vitro. Sovremennaya ortodontiya. 2015;3:57-60.
15. Gerashchenko SB, Chaykovsʹkiy YUB, Delʹtsova OÍ. Stovburoví klítini zuba. Galitsʹkiy líkarsʹkiy vísnik. 2011;18(4):5-8. [in Ukrainian].
16. Zorin NA, Zorina VN, Zorina RM, Levchenko VG. Universalʹnyy regulyator – a2 -makroglobulin. Klinich. lab. diagnostika. 2004;11:18-21.
17. Karyagina IYU, Zarembskiy RA, Balyabina MD. Ispolʹzovaniye metoda kompleksnogo opredeleniya aktivnosti tripsinopodobnykh proteinaz, a1 -antitripsinai a2 -makroglobulina v gastroenterologicheskoy klinike. Lab. delo. 1990;2:10-3.

«Bulletin of problems biology and medicine» Issue 3 (166), 2022 year, 501-509 pages, index UDK 612.015.13:616.314.17-002.1-085.361-092.9

10.29254/2077-4214-2022-3-166-501-509