Drehval O. A., Zubareva I. M., Havryliuk V. G., Lavrentieva K. V., Sklyar T. V.

VIABILITY AND FUNGISTATIC ACTIVITY OF TRICHODERMA VIRIDE DURING STORAGE IN PEAT

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About the author:

Drehval O. A., Zubareva I. M., Havryliuk V. G., Lavrentieva K. V., Sklyar T. V.

Heading:

MICROBIOLOGY

Type of article:

Scientific article

Annotation:

Micromycetes of the genus Trichoderma are widely used in plant protection against mycosis pathogens due to their broad spectrum of antagonistic activity and diverse mechanisms of action on phytopathogens. The process of deep cultivation of the fungus, compared to the surface one, is characterized by less laboriousness, it is easier to con trol and automate. The biomass of the fungus grown in deep conditions can be introduced into various substrates, such as composted manure, pulp, biochar, wood pellets, talc and peat, and used against soil phytopathogens by pre-sowing treatment of seeds or by applying the preparation to the root zone before planting plants. It is advisable to produce such forms of the preparation if they have a guaranteed storage of at least 6 months. The storage of three variants of the peat form of the preparation based on Trichoderma viride КМV-F-15, grown in deep conditions in three different media, was studied. It was found that during storage of all variants of the prepa ration at a temperature of 29°C (accelerated test), the viability and fungistatic activity of the fungus did not signifi cantly decrease for 6 months, and at room temperature, which simulates storage in an industrial warehouse, for 15 months. The number of CFU/g of the preparation at the end of the storage period remained greater than 1×106, which is considered sufficient for the manifestation of biological activity. The fungistatic activity of a 1.0% solution of the peat form of the preparation T. viride КМВ-F-15 against the soil pathogen of grain crops Fusarium culmorum remained higher than 60% throughout the entire storage period. No significant effect of the composition of the nutrient medium for the accumulation of fungal biomass in deep conditions on its storage in peat was established.

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Bibliography:

1. Rush TA, Shrestha HK, Gopalakrishnan Meena M, Spangler MK, Ellis JC, Labbé JL, et al. Bioprospecting Trichoderma: A systematic roadmap to screen genomes and natural products for biocontrol applications. Front Fungal Biol. 2021;2:716511. DOI: 10.3389/ ffunb.2021.716511.
2. Kopilov EP, Pavlenko AA, Tsekhmister GV, Kyslynska AS. Antagonistic activity of a new strain of Trichoderma viride and its effect on micro mycetes population in the root zone of corn plants. Silskohospodarska mikrobiolohiya. 2020;31:16-26. DOI: 10.35868/1997-3004.31.1625.
3. Oszust K, Pylak M, Frąc M. Trichoderma-based biopreparation with prebiotics supplementation for the naturalization of raspberry plant rhizosphere. Int J Mol Sci. 2021;22:6356. DOI: 10.3390/ijms22126356.
4. Modrzewska M, Blaszczyk L, Stepien L, Urbaniak M, Waskiewicz A, Yoshinari T, et al. Trichoderma versus Fusarium-inhibition of pathogen growth and mycotoxin biosynthesis. Mollecules. 2022;27(23):8146. DOI: 10.3390/molecules27238146.
5. Sood M, Kapoor D, Kumar V, Sheteiwy MS, Ramakrishnan M, Landi M, et al. Trichoderma: The “Secrets” of a multitalented biocontrol agent. Plants. 2020;9(6):762. DOI: 10.3390/plants9060762.
6. Sridharan AP, Thankappan S, Karthikeyan G, Uthandi S. Comprehensive profiling of the VOCs of Trichoderma longibrachiatum EF5 while interacting with Sclerotium rolfsii and Macrophomina phaseolina. Microbiol Res. 2020;236:126436. DOI: 10.1016/j.micres.2020.126436.
7. Pavlenko AA, Kopylov YeP, Cexmister GV Efektyvnist zastosuvannya shtamu Trichoderma viride z vysokoyu anatgonistychnoyu ta celyu lolitychnoyu aktyvnistyu. Silskogospodarska mikrobiologiya. 2021;33:88-95. DOI: 10.35868/1997-3004.33.88-95. [in Ukrainian].
8. Rimkus A, Namina A, Dzierkale MT, Grigs O, Senkovs M, Larsson S. Impact of growth conditions on the viability of Trichoderma asperellum during storage. Microorganisms. 2023;11(4):1084. DOI: 10.3390/ microorganisms11041084.
9. Martinez Y, Ribera J, Schwarze FWMR, De France K. Biotechnological development of Trichoderma-based formulations for biological control. Appl Microbiol Biotechnol. 2023;107(18):5595-5612. DOI: 10.1007/s00253-023-12687-x.
10. Unsoed P, As Y, Sumadi S, Simarmata T. Viability of Trichoderma harzianum grown on different carrier formulation. KnE Life Sciences: 2nd International Conference on Sustainable Agriculture and Food Security: A Comprehensive Approach (ICSAFS). 2017;2017:95-101. DOI: 10.18502/kls. v2i6.1024.
11. Senkovs M, Dzierkale MT, Rimkus A, Grigs O, Nikolajeva V. Application of a posttreatment to improve the viability and antifungal activity of Trichoderma asperellum biomass obtained in a bioreactor during submerged cultivation. Biology (Basel). 2022;11(11):1610. DOI: 10.3390/ biology 11111610.
12. Suherah, Kuswinanti T, Rosmana A, Rasyid B. The effect of organic medium use in formulation of Trichoderma harzianum and Pleurotus ostreatus in viability and decomposition of cacao pod husks waste. Pak J Biotechnol. 2018;15(1):95-100.
13. Prasad RD, Rangeshwaran R, Anuroop CP, Phanikumar PR. Bioefficacy and shelf life of conidial and chlamydospore formulations of Trichoderma harzianum Rifai. J Biol Control. 2002;16:145-148. DOI: 10.18311/jbc/2002/ 4124.
14. Rozhkova TO, Bilyavska LO. Antahonistychna aktyvnist hrybiv Gliocladium sp. v umovakh in vitro. Silskohospodarska mikrobiolohiya. 2022;36:55-63. DOI: 10.35868/1997-3004.36.55-63. [in Ukrainian].
15. Błaszczyk L, Ćwiek-Kupczyńska H, Hoppe Gromadzka K, Basińska-Barczak A, Stępień Ł, Kaczmarek J, et al. Containment of Fusarium culmorum and its mycotoxins in various biological systems by antagonistic Trichoderma and Clonostachys strains. J Fungi. 2023;9(3):289. DOI: 10.3390/jof9030289.
16. Wagacha JM, Muthomi JW. Fusarium culmorum: infection process, mechanisms of mycotoxin production and their role in pathogenesis in wheat. Crop Protection. 2007;26(7):877-885. DOI: 10.1016/j.cropro.2006. 09.003.
17. Khemir E, Chekali S, Moretti A, Gharbi MS, Allagui MB, Gargouri S. Impacts of previous crops on inoculum of Fusarium culmorum in soil, and development of foot and root rot of durum wheat in Tunisia. Phytopathol Mediterr. 2020;59(1):187-201. DOI: 10.36253/phyto-10827.

Publication of the article:

«Bulletin of problems biology and medicine», 2026 Issue 1, 180, 398-408 pages, index UDC 579.64 + 632.9

DOI:

10.29254/2077-4214-2026-1-180-398-408