Tverdokhlib I. V., Kobeza P. A.

THE INFLUENCE OF CHRONIC HYPOXIA ON THE PRENATAL DEVELOPMENT OF THE CONTRACTILE APPARATUS OF RAT CARDIOMYOCYTES

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About the author:

Tverdokhlib I. V., Kobeza P. A.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

There is a wide range of publications that analyze various aspects of the influence of prenatal hypoxia on ontogenetic transformations of the myocardium, however, the topological features of changes in the contractile apparatus of a cardiomyocyte that is formed and develops under conditions of chronic prenatal hypoxia require detailed study. The aim is establish the ultrastructural features of changes in the contractile apparatus of typical cardiomyocytes of different parts of the embryonic myocardium of rats under conditions of chronic hypoxia. The object of the study were the hearts of rat embryos obtained after the administration of sodium nitrite to pregnant females at a dose of 50 mg/kg daily from the 10th to the 20th day of gestation. The material was analyzed by transmission electron microscopy in 12 topographical localizations of the myocardium. Results. It has been established that hypoxia inhibits the formation of sarcomeres, leads to disorganization of myofibrils and mitochondrial damage. Starting from the 17th day of development, progressive destruction of contractile structures is observed: fragmentation of myofibrils, disruption of intercalated discs and disintegration of filaments. The greatest changes were recorded in the intramural and subendocardial zones of the ventricles. In the atria, hypoxia caused a restriction of the spatial organization of myofibrils without their pronounced lysis. Chronic prenatal hypoxia leads to inhibition of sarcomerogenesis and disorientation of myofibrils against the background of crystallolysis and swelling of mitochondria of typical cardiomyocytes mainly in the subendocardial zone of both ventricles and the interventricular septum. Starting from the 17th day of prenatal ontogenesis, the effect of chronic hypoxia is accompanied by fragmentation of newly formed myofibrils, dissociation of sarcomeres, splitting of telophragms and intercalated discs in the intramural and subepicardial zones of both ventricles.

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Publication of the article:

«Bulletin of problems biology and medicine», 2025 Issue 3,178, 419-432 pages, index UDC 612.17 576.3 616-092.9 591.3.036.8

DOI:

10.29254/2077-4214-2025-3-178-419-432