Sіusіuka V. H., Kyrychenko M. M.

ASSESSMENT OF BIOCHEMICAL MARKERS OF OXIDATIVE STRESS IN WOMEN WITH HYPERTENSIVE DISORDERS OF PREGNANCY

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About the author:

Sіusіuka V. H., Kyrychenko M. M.

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CLINICAL AND EXPERIMENTAL MEDICINE

Type of article:

Scientific article

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Hypertensive disorders of pregnancy (HDP) are major contributors to maternal morbidity and adverse perinatal outcomes and are linked to oxidative stress (OS). Evaluating OS biomarkers in HDP may refine pathophysiologic understanding and risk assessment. The aim of the study – to evaluate biochemical markers of OS in women with HDP. Prospective controlled study of 65 third-trimester women: HDP group (n=35; gestational hypertension or moderate-to-severe preeclampsia) and controls (n=30). In plasma, protein oxidative modification was measured: aliphatic aldehyde-protein hydrazones (AFG) and carbonyl-protein hydrazones (CFG). Antioxidant enzymes were assessed: superoxide dismutase (SOD) and glutathione reductase (GR). Data are median [Q1-Q3]. Shapiro-Wilk, Mann-Whitney U (between-group), and Wilcoxon signed-rank (paired) tests were used; significance p<0.05. In the HDP group, AFG increased from 4.28 to 6.86 (p<0.05) and CFG from 3.60 to 4.76 (p<0.05) after stimulation. In controls, AFG rose from 2.93 to 4.14 (p<0.05), whereas CFG changed from 2.98 to 3.26 (p>0.05). Between-group differences in AFG and CFG were significant before and after stimulation (p<0.05). Antioxidant enzymes were reduced in HDP: SOD 7.89 [6.17-9.09] vs 13.78 [10.99-16.52] U/mg protein/min (p<0.05); GR 6.70 [6.30-6.95] vs 18.20 [17.03-18.80] μmol/g protein/min (p<0.05). HDP is characterized by heightened protein susceptibility to free-radical damage and reduced enzymatic antioxidant defense (SOD, GR), supporting a central role of oxidative stress in pathogenesis. Combined protein oxidative modification and enzymatic indices may aid prognostication and management in HDP.

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Publication of the article:

«Bulletin of problems biology and medicine», 2025 Issue 3,178, 237-249 pages, index UDC 618.3:618.36-007.1:577.15:612.015.3

DOI:

10.29254/2077-4214-2025-3-178-237-249