Poniatovskyi V. A., Shyrobokov V. P., Linenko О. М., Liutko О. B.

PROSPECTS FOR THE USE OF AUTOLOGOUS FIBRIN GLUE WITH BACTERIOPHAGES IN TRAUMATOLOGY AND ORTHOPEDICS

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Poniatovskyi V. A., Shyrobokov V. P., Linenko О. М., Liutko О. B.

Heading:

MICROBIOLOGY

Type of article:

Scientific article

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Injectable platelet-rich fibrin glue (i-PRF) is an autologous platelet concentrate with fibrin fibers, widely used in various fields of medicine and recognized as a valuable adjunct for tissue regeneration during surgical procedures. In addition, i-PRF can serve as a vehicle for the local delivery and depot of antibacterial agents. The incorporation of bacteriophages into fibrin glue may offer an additional tool in the fight against antibiotic-resistant microbial strains. The aim of the study is to assess the biocompatibility and stability of various types of bacteriophages incorporated into polymerized i-PRF, to study the dynamics of their release from fibrin scaffolds, and to determine the infectious activity of desorbed bacteriophages. A high-concentration bacteriophage cocktail (≥10¹⁰ PFU/mL) containing different types of bacteriophages was prepared using ultrafiltration. The injectable autologous fibrin glue was polymerized in the presence of the prepared phage cocktail, after which fibrin blocks were placed in saline and incubated at 36±1 °C for 7 days to enable bacteriophage desorption. At designated time points, the concentration of desorbed phages in the saline solution was quantified, and their lytic activity was evaluated. Fibrin glue polymerized in the presence of the bacteriophage cocktail targeting five bacterial species consistently released all phage types over a 7-day incubation period in liquid medium at 36±1 °C. Using the agar overlay method, it was confirmed that the desorbed bacteriophages caused specific lysis of all five tested microorganisms (Escherichia coli, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Streptococcus pyogenes) throughout the observation period. The findings support the feasibility of using injectable autologous fibrin glue as a matrix for sustained local delivery of bacteriophages. This approach holds promise for the prevention and treatment of infectious complications in traumatology and orthopedics.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», Issue 2,177, 401-415 pages, index UDC 616-089.843:578.2:616-002-022.7:616.7

DOI:

10.29254/2077-4214-2025-2-177-401-415