Feskov O. M., Zhylkova Ye. S., Somova O. V., Feskova A. O., Konoplia L. A.

CLINICAL OUTCOME ASSOCIATED WITH ASSISTED REPRODUCTIVE TECHNOLOGIES IN COUPLES WITH VARIOUS FACTORS OF INFERTILITY AFTER TRANSFER OF FROZEN EMBRYOS WITH A BALANCED KARYOTYPE

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About the author:

Feskov O. M., Zhylkova Ye. S., Somova O. V., Feskova A. O., Konoplia L. A.

Heading:

CLINICAL AND EXPERIMENTAL MEDICINE

Type of article:

Scientific article

Annotation:

One of the main conditions to get a healthy pregnancy in in vitro fertilization cycles is the transfer of an embryo with a balanced karyotype into the uterine cavity. Such a transfer is possible only within the framework of the cryo protocol when the frozen embryos are used. Currently, the question if the oocyte or sperm influences on the process of blastocyst formation and the presence of aneuploidy in embryos at the early stages of development, when in vitro fertilization cycle is carried out, is still open. The aim of this work was to determine the rates of formation for blas tocysts with a balanced karyotype and the clinical pregnancy rates after the transfer of frozen embryos for couples with male and tubal-peritoneal infertility factors. In particular, the influence of such parameters of male fertility as concentration, motility and morphology of spermatozoa in the ejaculate on the processes of early embryogenesis was investigated. Totally, preimplantation genetic testing for aneuploidy (PGT-A) was performed for 613 blastocysts received from 156 couples with male and tubo-peritoneal infertility factors and using donor gametes. The PGT-A was carried out by the method of next generation sequencing. Spermogram parameters were assessed according to WHO recommendations from 2010. Embryos were obtained using assisted reproductive technologies. Blastocysts freezing was performed using the vitrification method. Statistical hypotheses were tested at significance levels of 0.05, 0.025, 0.01. The relationship between the morphology and motility of spermatozoa in the ejaculate in men with spermatogenesis failures and the rate of formation of morphologically high quality blastocysts has been proven (r=0.24 and r=0.21, respectively). A decrease in spermogram parameters leads to a decrease in the in vitro fertil ization rates of mature oocytes at the MII stage (p<0.05). It has been confirmed that advanced matermity age is associated with an increase in the formation of aneuploid embryos when using assisted reproductive technologies (p<0.05). A balanced embryo karyotype is one of the most important conditions for pregnancy outcome in couples with different factors of infertility.

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Publication of the article:

«Bulletin of problems biology and medicine», Issue 2,177, 268-276 pages, index UDC 618.177-089.888.11:611.013:57.043

DOI:

10.29254/2077-4214-2025-2-177-268-276