Rieznichenko L. S., Gruzina T. G., Podolska V. I., Yakubenko L. M., Gryshchenko N. I., Vitrak K. V., Liutko O. B., Dybkova S. M.

INVESTIGATION OF ANTAGONISTIC ACTIVITY OF METAL NANOPARTICLES (Au, Ag, Fe) COMPOSITION WITH PROBIOTIC STRAIN Lactobacillus plantarum FOR THE DEVELOPMENT OF METAL-PROBIOTICS

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Rieznichenko L. S., Gruzina T. G., Podolska V. I., Yakubenko L. M., Gryshchenko N. I., Vitrak K. V., Liutko O. B., Dybkova S. M.

Heading:

MICROBIOLOGY

Type of article:

Scientific article

Annotation:

Metal nanoparticles are widely used in medicine, veterinary medicine, and biotechnology. The aim of the study was to investigate the antagonistic activity of metal-containing compositions based on the probiotic strain Lactobacillus plantarum with gold, silver and iron nanoparticles against the test cultures Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29213, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Serratia marcescens, Escherichia coli 055, Enterobacter aerogenes, Acinetobacter baumannii, Staphylococcus aureus 209, Staphylococcus epidermidis, Klebsiella pneumoniae, Salmonella typhimurium, Shigella sonnei, Shigella flexneri, Proteus mirabilis, Proteus vulgaris, Candida albicans. Colloidal-chemical, biochemical and microbiological methods were used in the study. Compositions of metal nanoparticles with L. plantarum cells (1×109 CFU/ml): (1) a composition with a mixture of Au/Ag/Fe nanoparticles 0.32 Au/160.0 Ag / 100.0 Fe μg/ml; (2) a composition with 30 nm silver nanoparticles (160.0 μg/ml); and (3) a composition with 4 nm NBC silver nanoparticles (content of Ag 8×10-5 μg/ml) were developed and their effective antagonistic effect against all studied test cultures of microorganisms was established. Plasmid-eliminating effect of the developed compositions containing silver nanoparticles was revealed which gives the ability to prevent the transfer of antibiotic resistance genes to the host intestinal commensals.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 4, 175, 525-532 pages, index UDC 577.1:616-003.269:636

DOI:

10.29254/2077-4214-2024-4-175-525-532