Kirichek P. V., Lukyantseva H. V.

CHANGES IN THE SECRETORY ACTIVITY OF MAST CELLS UNDER THE INFLUENCE OF BIOLOGICALLY ACTIVE SUBSTANCES

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About the author:

Kirichek P. V., Lukyantseva H. V.

Heading:

MORPHOLOGY

Type of article:

Scientific article

Annotation:

Mastocytes are highly specialised, multifunctional cells with basophilic granules in the cytoplasm. These heterogeneous myeloid cells are present in vascularised tissues, acting as immune mediators, responding to invasion by antigen carriers, participating in the organisation of inflammatory reactions and tissue remodelling, etc. The scientific literature contains very little information on mastocytes' response to the effects of biologically active substances involved in the regulation of colon motility, which prompted us to conduct our study. The aim was to investigate the peculiarities of changes in the secretory activity of mastocytes of subcutaneous adipose tissue and mesentery of rats under the influence of biologically active substances. The study was conducted on white outbred mature male rats; morphometric analysis was used to assess the secretory activity of mastocytes. Preparations of subcutaneous adipose tissue and mesentery were prepared. The condition of mastocytes in the preparations was assessed by light microscopy, the degree of secretory activity was assessed by the degranulation index, the intensity of degranulation was assessed by degrees (weak, moderate, strong). Some of the experiments were performed in vivo, others in vitro. The effect of flocalin, foridone, a mixture of flocalin and foridone, NH4Cl solution, quercetin, and caffeine on mast cell secretion was studied. The maximum effect of stimulation of mast cell secretion was recorded with a mixture of flocalin and foridone, and the lowest effect was recorded with flocalin alone. This trend is characteristic of both subcutaneous adipose tissue and mesentery, and is also inherent in in vivo and in vitro conditions. The stabilising effect of quercetin and caffeine on mast cell secretion was characteristic of mesenteric and subcutaneous adipose tissue mast cells in vivo and in vitro. The lowest degree of changes in mast cell secretion under the influence of the applied substances was recorded in preparations of subcutaneous adipose tissue compared to mesentery preparations. The greatest changes in mast cell secretion were observed in in vitro compared to the in vivo effect. All the selected biologically active substances were found to affect mast cell secretion. At the same time, flocalin, foridone, a mixture of both of these substances, and alkalisation of the medium proved to be stimulants of mast cell degranulation. Quercetin and caffeine, on the other hand, have an inhibitory effect on mast cell secretion.

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 3, 174, 280-289 pages, index UDC 612.11:613.292(045)

DOI:

10.29254/2077-4214-2024-3-174-280-289