Smirnov I. V.

ASSESSMENT OF BEHAVIORAL REACTIONS IN RATS WITH A MODEL OF METABOLIC SYNDROME

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About the author:

Smirnov I. V.

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CLINICAL AND EXPERIMENTAL MEDICINE

Type of article:

Scientific article

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In the experiment, a model of metabolic syndrome (MS) was reproduced in white Wistar rats of outbred breeding (12 months of age), and the peculiarities of the experimental animals' behavioural reactions, emotional state, and locomotor activity were studied. The animals were divided into two groups: Group 1 - 12 rats (intact animals - control), and Group 2 - 30 rats with a reproduced model of MS. The MS model was reproduced by keeping animals for 72 days on a standard diet with an additional 30 g of white bread per day. In the free consumption mode, they consumed a 10% fructose solution in distilled water as drinking water. The results of the study showed that the development of MS is accompanied by a suppression of the functional activity of the central nervous system (animals do not go to the centre of the "open field", the number of crossed squares, vertical stands and the number of peeps into holes are significantly reduced by an average of 26%, and the duration of stops increases by 165% compared to the control group) and hyperactivation of the ANS (a significant increase in the duration of each grooming act against the background of an increase in the number of defecations and urinations). The authors believe that the reproduced model of MS leads to the formation of persistent disorders of motor activity with concomitant disorders of emotional activity and autonomic state, and as a result, the formation of an imbalance in the cognitive functions of animals.

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1. Noubiap JJ, Nansseu JR, Lontchi-Yimagou Е, Nkeck JR, Nyaga UF, Ngouo АТ, et al. Geographic distribution of metabolic syndrome and its components in the general adult population: A meta-analysis of global data from 28 million individuals. Diabetes Research and Clinical Practice. 2022;188:109924. DOI: https://doi.org/10.1016/j.diabres.2022.109924.
2. Saklayen MG. The Global Epidemic of the Metabolic Syndrome. Curr Hypertens Rep. 2018;20(2):12. DOI: 10.1007/s11906-018-0812-z.
3. Silva-Ochoa AD, Velasteguí E, Falconí IB, García-Solorzano VI, Rendón-Riofrio A, Sanguña-Soliz GA, et al. Metabolic syndrome: Nutriepigenetic cause or consequence? Heliyon. 2023;9(11):21106. DOI: 10.1016/j.heliyon. 2023.e21106.
4. Cozma A, Sitar-Taut A, Orăşan O, Leucuta D, Alexescu T, Stan A, et al. Determining Factors of Arterial Stiffness in Subjects with Metabolic Syndrome. Metab Syndr Relat Disord. 2018;16(9):490-496. DOI: 10.1089/met.2018.0057.
5. Ghezzi AC, Cambri LT, Botezelli JD, Ribeiro C, Dalia RA, de Mello MA. Metabolic syndrome markers in wistar rats of different ages. Diabetol Metab Syndr. 2012;4(1):16. DOI: 10.1186/1758-5996-4-16.
6. Levchuk NI, Lukashenia OS, Kovzun OI. Eksperymentalne modeliuvannia metabolichnoho syndromu, indukovanoho diietoiu, u laboratornykh tvaryn. Endokrynolohiia. 2021;26(3):298-310. DOI: https://doi.org/10.31793/1680-1466.2021.26-3.298. [in Ukrainian].
7. Hozhenko AI, Hryshko MYu. Patohenetychni osnovy rozvytku ozhyrinnya yak naslidok funktsional’no-metabolichnoho dysbalansu v orhanizmi (ohlyad). Aktual’ni problemy transportnoyi medytsyny. 2019;1(55):29-40. Dostupno: https://repository.pdmu.edu.ua/items/ cb7b640c-7cdc-40c3-a3a1-2d6c53164b04. [in Ukrainian].
8. Fahed G, Aoun L, Bou Zerdan M, Allam S, Bou Zerdan M, Bouferraa Y, Assi HI. Metabolic Syndrome: Updates on Pathophysiology and Management in 2021. Int J Mol Sci. 2022;23(2):786. DOI: 10.3390/ijms23020786.
9. Głombik K, Detka J, Góralska J, Kurek A, Solnica B, Budziszewska B. Brain Metabolic Alterations in Rats Showing Depression-Like and Obesity Phenotypes. Neurotox Res. 2020;37(2):406-424. DOI: 10.1007/s12640-019-00131-w.
10. Lee TH, Yau SY. From Obesity to Hippocampal Neurodegeneration: Pathogenesis and Non-Pharmacological Interventions. Int J Mol Sci. 2020;22(1):201. DOI: 10.3390/ijms22010201.
11. Cuervo Sánchez ML, Prado Spalm FH, Furland NE, Vallés AS. Pregestational fructose-induced metabolic syndrome in Wistar rats causes sexually dimorphic behavioral changes in their offspring. Dev Neurobiol. 2024;84(3):142-157. DOI: 10.1002/dneu.22940.
12. Nasibullin BA, Hushchа SH, Oleshko OIa. Robota z laboratornymy tvarynamy: dohliad ta vidtvorennia modelei patolohichnykh staniv: posibnyk Odesa: Polihraf; 2023. 96 s. Dostupno: https://kurort.gov.ua/wp-content/uploads/2024/05/posibnuk-tvarunu.pdf. [in Ukrainian].
13. Levchuk NI, Lukashenya OS, Kovzun OI. Eksperymental’ne modelyuvannya metabolichnoho syndromu, indukovanoho diyetoyu, u laboratornykh tvaryn. Endokrynolohiya. 2021;26(3):298-310. DOI: 10.31793/1680‑1466.2021.26‑3.298. [in Ukrainian].
14. Walsh RN, Cummings RA. The open-field test: A critical Review. Psychological Bulletin. 1976;83:482-504.
15. Botsula IV, Kireyev I, Koshovyi OM, Mazur MO, Shebanov VA. Povedinkovi reaktsii hryzuniv pislia vvedennia pokhidnykh 1,2,3-tryazolo1,4-benzodiazepiniv v testi «vidkrytoho polia». Farmatsevtychnyi chasopys. 2023;(4);70-77. DOI: https://doi.org/10.11603/2312- 0967.2023.4.14297. [in Ukrainian].
16. Kysylevska A, Babov K, Gushcha S, Prokopovych I, Bezverkhniuk T. Qualimetric Model for Assessing the State of the Central Nervous System of Animals When Studying the Mechanism of Biological Activity for Mineral Waters. In: Tonkonogyi V, Ivanov V, Trojanowska J, Oborskyi G, Pavlenko I. (eds). Advanced Manufacturing Processes III. InterPartner 2021. Lecture Notes in Mechanical Engineering; 2021; Springer, Cham; 2022. p. 424-434. DOI: https://doi.org/10.1007/978-3-030-91327-4_42.
17. Koshovyi O, Raal A, Kireyev I, Tryshchuk N, Ilina T, Romanenko Y, et al. Phytochemical and Psychotropic Research of Motherwort (Leonurus cardiac L.) Modified Dry Extracts. Plants (Basel). 2021;10(2):230. DOI: 10.3390/plants10020230.
18. Babov KD, Gushcha SG, Nasibullin BA, Badiuk NS. Polshchakova TV, Zabolotna IB. Development and pathogenetic substantiation of the model of post-traumatic stress disorder. PhOL–PharmacologyOnLine. 2020;3:284-291. Avaіlable from: https://pharmacologyonline.silae.it/ files/archives/2020 /vol3/PhOL_2020_3_A030_Babov.pdf.
19. Kalueff AV, Stewart AM, Song C, Berridge KC, Graybiel AM, Fentress JC. Neurobiology of rodent self-grooming and its value for translational neuroscience. Nat Rev Neurosci. 2016;17(1):45-59. DOI: 10.1038/nrn. 2015.8.
20. Yavtushenko I, Levkov A, Kostenko V. Zminy povedinkovykh reaktsii shchuriv pislia vidtvorennia cherepno-mozkovoi travmy ta yikhnia korektsiia moduliatoramy transkryptsiinykh chynnykiv. Aktualni problemy suchasnoi medytsyny: Visnyk Ukrainskoi medychnoi stomatolohichnoi akademii. 2020;20(4):157-162. DOI: https://doi.org/10.31718/2077-1096. 20.4.157. [in Ukrainian].
21. Wikgren J, Nokia MS, Mäkinen Е, Koch LG, Britton SL, Kainulainen Н, et al. Rats with elevated genetic risk for metabolic syndrome exhibit cognitive deficiencies when young. Physiology & Behavior. 2021;236:113417. DOI: https://doi.org/10.1016/j.physbeh.2021.113417.
22. Rebolledo-Solleiro D, Roldán-Roldán G, Díaz D, Velasco M, Larqué C, Rico-Rosillo G, et al. Increased anxiety-like behavior is associated with the metabolic syndrome in non-stressed rats. PLoS One. 2017;12(5):e0176554. DOI: 10.1371/journal.pone.0176554.
23. Faria-Pereira A, Morais VA. Synapses: The Brain’s Energy-Demanding Sites. Int J Mol Sci. 2022;23(7):3627. DOI: 10.3390/ijms23073627.
24. Clemente-Suárez VJ, Beltrán-Velasco AI, Redondo-Flórez L, Martín-Rodríguez A, Yáñez-Sepúlveda R, Tornero-Aguilera JF. NeuroVulnerability in Energy Metabolism Regulation: A Comprehensive Narrative Review. Nutrients. 2023;15(14):3106. DOI: 10.3390/ nu15143106.
25. Sharma S, Fulton S. Diet-induced obesity promotes depressive-like behaviour that is associated with neural adaptations in brain reward circuitry. Int J Obes. 2013;37:382-389. DOI: https://doi.org/10.1038/ijo.2012.48.
26. Yamada N, Katsuura G, Ochi Y, Ebihara К, Kusakabe Т, Hosoda К, et al. Impaired CNS Leptin Action Is Implicated in Depression Associated with Obesity. Endocrinology. 2011;152(7):2634-2643. DOI: https://doi.org/10. 1210/en.2011-0004.

Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 3, 174, 197-203 pages, index UDC 616-008.9-092.9

DOI:

10.29254/2077-4214-2024-3-174-197-203