Chekanova V. V., Hvozdiuk Ya. V., Pakhomova Yu. S., Kompaniets A.M.

PHYSICO-CHEMICAL PROPERTIES AND CRYO PROTECTIVE EFFECT OF COMBINED ENVIRONMENTS BASED ON 1,2-PROPANEDIOL IN COMBINATION WITH POLYVINYL ALCOHOL MOLECULAR WEIGHTS 9 kDa

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Chekanova V. V., Hvozdiuk Ya. V., Pakhomova Yu. S., Kompaniets A.M.

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BIOLOGY

Type of article:

Scientific article

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The physico-chemical properties of combined media containing PVA with a molecular weight of 9 kDa and 20- 40% solutions of cryoprotectant 1,2-propanediol (1,2-PD) were studied to characterize hydrophilic-hydrophobic interactions in these solutions. From the list of physicochemical properties of cryoprotectant solutions, dynamic viscosity and surface activity are of particular interest to cryobiologists. The surface activity of cryoprotectant solutions, which is determined at the boundary of the water-nonpolar media phase distribution, gives an idea of the ability of substances to adsorb on the surface of the cell membrane; dynamic viscosity of cryoprotectant solutions - about the interaction of substances with water. It was established that the viscosity and density indicators increase with the increase in the concentration of 1,2-PD and PVA, which, in turn, indicates an increase in the hydrophilicity of the combined media. The surface tension index of the combined media decreased due to the change in the structure of 1,2-PD-PVA, which leads to a decrease in the hydrophobicity of these media. Combined media containing 1,2-PD and PVA can form micelles in the liquid phase. Cryoprotective media of different composition based on the combination of endocellular 1,2-propanediol (1,2- PD) with solutions of polyvinyl alcohol (PVA) during freezing of erythrocytes were studied. After freezing-thawing of erythrocytes in multicomponent solutions based on 1,2-PD with PVA of molecular weight 9 kDa, there is a tendency to increase the preservation of erythrocytes as a result of hemolysis in comparison with the effect of 20%, 30%, and 40% monosolutions of 1,2-PD. When erythrocytes were frozen in solutions containing a combination of PVA with the cryoprotectant 1,2-PD, at a slow controlled speed (3ºC/min.), the highest cell preservation rates were obtained for the media of 30% 1,2-PD +0.2% PVA This solution made it possible to preserve 89% of erythrocytes with low osmotic fragility. The preservation of erythrocytes depends on the composition of the combined media: on the molecular weight of PVA, the concentration of solutions of individual cryoprotectants and PVA.

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 3, 174, 98-107 pages, index UDC 612.111:57.086.13:547.422+678.744

DOI:

10.29254/2077-4214-2024-3-174-98-107