Hyriavenko N. I., Sumtsov D. G., Sikora K. O., Lуndіna Y. S., Shamrai A. V., Lуndіn M. S., Romaniuk A. M.

INFLUENCE OF THE IMMUNE MICROENVIRONMENT ON THE COURSE AND PROGRESSION OF PRIMARY FALLOPIAN TUBE CANCER

---

Show/Download

About the author:

Hyriavenko N. I., Sumtsov D. G., Sikora K. O., Lуndіna Y. S., Shamrai A. V., Lуndіn M. S., Romaniuk A. M.

Heading:

PATHOMORPHOLOGY

Type of article:

Scientific article

Annotation:

Among all malignancies, primary fallopian tube carcinoma is the most insidious and challenging to diagnose and treat appropriately. The tumor microenvironment plays a crucial role in supporting the functioning of cancer cells and actively influences them. Understanding the prognostic potential of the presence of tumor-infiltrating immune cells is important not only for its intensity but also for the subpopulation composition of the infiltrating cells. Objective - to evaluate the qualitative composition of the immune microenvironment of tissue in primary serous carcinoma of the fallopian tubes. The study was conducted on 66 samples of tumor tissue from fallopian tubes. Molecular and biological markers used in the study included Ki-67, p53, VEGF, ER, PR, CD3, CD79α, CD68, and myeloperoxidase (MPO). The criteria for assessing molecular and biological markers were conducted according to commonly accepted methods and according to the manufacturer’s protocol. The presence of tumor-infiltrating inflammatory infiltrate was established in 40.9% of primary serous carcinoma of the fallopian tubes. The presence and intensity of infiltration depended on the degree of atypia of cancer cells (p<0.05). The cellular component of the microenvironment of primary serous carcinoma of the fallopian tubes is predominantly represented by B-lymphocytes. A decrease in the level of T-cell infiltration in tumor tissue was somewhat associated with lymph node metastasis (p<0.001). The presence of macrophages in tumor tissue was significantly higher in the group of patients with primary serous carcinoma of the fallopian tubes with metastasis to regional lymph nodes (p<0.001). A moderate negative correlation was found between the presence of T-lymphocytes in the tumor microenvironment and ER (r=-0.48, p<0.001) and PR (r=-0.43, p<0.001). A negative correlation of moderate strength was established between the presence of B-lymphocytes, ER (r=- 0.56, p<0.001), and PR (r=- 0.50, p<0.001). A correlation was found between the presence of T- and B-lymphocytes in the tumor microenvironment and Ki-67 expression (r=0.28, p<0.05 and r=0.27, p<0.05 respectively). A moderate correlation was established between the presence of T-lymphocytes (r=0.47, p<0.001), B-lymphocytes (r=0.58, p<0.001), and p53 expression. No statistically significant correlation was found between the presence of T-lymphocytes, B-lymphocytes, macrophages in the tumor microenvironment, and VEGF expression (p>0.05). The presence of inflammatory tumor microenvironment predominantly localizes in low-grade neoplasms (66.7%), and its qualitative and quantitative composition is associated with decreased expression of steroid hormone receptors in tumor tissue (p<0.001) and correlates with Ki-67 (p<0.05) and p53 (p<0.001) expression. Decreased levels of CD3+ T-lymphocytes in primary fallopian tube carcinoma tissue and significant infiltration of the tumor by CD68+ macrophages are associated with regional metastasis and are indicators of an unfavorable prognosis.

Tags:

Bibliography:

1. Siegel RL, Miller KD, Wagle NS, Jemal A. Cancer statistics, 2023. CA Cancer J Clin. 2023;73(1):17-48. DOI: 10.3322/caac.21763.
2. Khromchenko K, Aikman N, Sun X, ElSahwi K. Early-stage serous fallopian tube carcinoma. BMJ Case Rep. 2023;16(11):e255638. DOI: 10.1136/bcr-2023-255638.
3. Maeda M, Hisa T, Matsuzaki S, Ohe S, Nagata S, Lee M, et al. Primary Fallopian Tube Carcinoma Presenting with a Massive Inguinal Tumor: A Case Report and Literature Review. Medicina (Kaunas). 2022;58(5):581. DOI: 10.3390/medicina58050581.
4. Vats B, Yadav R, Parihar M, Jaiswal N, Chatterjee P, Osama MA. Primary fallopian tube endometroid carcinoma: A rare case report. J Cancer Res Ther. 2023;19(2):S898-S900. DOI: 10.4103/jcrt.jcrt_78_22.
5. Reade CJ, McVey RM, Tone AA, Finlayson SJ, McAlpine JN, Fung-Kee-Fung M, et al. The fallopian tube as the origin of high grade serous ovarian cancer: review of a paradigm shift. J Obstet Gynaecol Can. 2014;36(2):133-40.
6. Kim J, Coffey DM, Creighton CJ, Yu Z, Hawkins SM, Matzuk MM. High-grade serous ovarian cancer arises from fallopian tube in a mouse model. Proc Natl Acad Sci U S A. 2012;109(10):3921-6.
7. Stewart KT, Hoang L, Kwon JS. Serous tubal intraepithelial carcinoma (STIC) outcomes in an average risk population. Gynecol Oncol Rep. 2024;51:101334. DOI: 10.1016/j.gore.2024.101334.
8. Margul D, Yu C, AlHilli MM. Tumor Immune Microenvironment in Gynecologic Cancers. Cancers (Basel). 2023;15(15):3849. DOI: 10.3390/cancers15153849.
9. Romaniuk A, Lyndin M. Immune microenvironment as a factor of breast cancer progression. Diagn Pathol. 2015;10:79. DOI: 10.1186/s13000-015-0316-y.
10. Zhang H, Wang Y, Liu C. A novel autophagy-related gene signature associated with prognosis and immune microenvironment in ovarian cancer. Cancer Immunol Immunother. 2024;73(5):853-865. DOI: 10.1007/s00262-024-03108-9.
11. Dahlberg CI, Sarhan D, Chrobok M, Duru AD, Alici E. Natural Killer Cell-Based Therapies Targeting Cancer: Possible Strategies to Gain and Sustain Anti-Tumor Activity. Front Immunol. 2015;6:605. DOI: 10.3389/fimmu.2015.00605.
12. Smith J, Jones A, Davis B. Roles of immune microenvironment in the female reproductive maintenance and regulation: novel insights into the crosstalk of immune cells. Reprod Biol Endocrinol. 2024;22(1):58. DOI: 10.1186/s12958-024-00820-6.

Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 2, 173, 433-437 pages, index UDC 618.19-006.6-07:616-006.6-073.7

DOI:

10.29254/2077-4214-2024-2-173-433-437