Bilookyi O. V., Bilookyi V. V., Proniaiev D. V., Proniaiev V. V.

POLARIZATION MAPPING AND STATISTICAL ANALYSIS OF LAYER MAPS OF POLARIZATION AZIMUTHS OF MICROSCOPIC IMAGES OF BLOOD FACES IN THE DIFFERENTIAL DIAGNOSTICS OF THYROID GLAND DISEASES

---

Show/Download

About the author:

Bilookyi O. V., Bilookyi V. V., Proniaiev D. V., Proniaiev V. V.

Heading:

METHODS AND METHODOLOGIES

Type of article:

Scientific article

Annotation:

Among the numerous biomedical diagnostic applications of soft tissue structure, Mueller matrix polarimetry of the optical anisotropy of biological tissues plays an important role. Various systems of Mueller matrix polarimetry have been developed to solve these diagnostic problems. The work is aimed at using the method of layer-by-layer polarisation-interference mapping of dehydrated blood films to determine new objective criteria (markers) for minimally invasive differential diagnosis of thyroid pathology. A comparative analysis of the effectiveness of methods of multifractal processing of polarisation ellipticity maps of digital microscopic images of supramolecular protein networks of polycrystalline blood fractions was carried out in order to determine new objective criteria (markers) for digital laser histological differential diagnosis of thyroid pathology. The results of the statistical analysis of the data of the polarisation-interference mapping method with digital holographic reproduction of phase maps of the polarisation azimuth were determined. From a biophysical point of view, the results correlate well with the model representations and prognostic scenarios of pathological changes in the polycrystalline structure of supramolecular networks of blood phases of donors and patients from different study groups. In this case, the algorithmically reconstructed layer maps α(ϑ,m,n) are "devoid" of the distorting effect of the depolarised background and are almost uniquely related to the optically anisotropic properties of the polycrystalline structure of blood fractions. Therefore, as the concentration of chiral optically active structures increases ("normal - nodular goiter - autoimmune thyroiditis - papillary cancer"), the dynamics of changes in the mean and variance of layer-by-layer distributions of the polarisation azimuth α(ϑ,m,n) increases in comparison with the data of integrated polarisation mapping of digital microscopic images of blood phases. As a result of the study, the most optimal markers of the method of integrated polarisation mapping of azimuths of polarisation of digital microscopic images of blood phases were identified and a good level (~87%-89%) of balanced accuracy of differential diagnosis of thyroid pathologies was established. The results of polarization-interference mapping of microscopic images with digital holographic reproduction of layer-by-layer coordinate distributions of random values of the azimuth of blood phase polarization are presented and analysed within the framework of the statistical approach.

Tags:

Bibliography:

1. Kolobrodov VG. Tymchyk GS, Kolobrodov MS, Vasyura AS, Komada P, Azeshova Z. The output signal of a digital optoelectronic processor. Processing of Conference Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments; 2018; Wilga. Bellingham: SPIE.digitallibrary; 2018. p. 108080W. Available from: https://www.spiedigitallibrary.org/conference-proceedings-ofspie/10808/ 2501595/The-output-signal-of-a-digital-optoelectronic-processor/10.1117/ 12.2501595.full#_=_.
2. Kolobrodov VG, Dobrovolska CV, Mykytenko VI, Tymchik GS, Tiagur VM, Komada P, et al. Spaceborne linear array imager’s spatial resolution for arbitrary viewing angles. Processing of Conference Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments; 2017; Wilga. Bellingham: SPIE.digitallibrary; 2017. p. 10445. Available from: https://www. spiedigitallibrary.org/conference-proceedings-of-spie/10445/104450J/Spaceborne-linear-array-imagers-spatial-resolution-for-arbitraryviewing-angles/10.1117/12.2280909.full#_=_.
3. Tymchik G, Skytciouk VI, Klotchko TR. Distortion of Phantom Object’s Realizations in Biological Presence Zone. Processing of 2020 IEEE 40th International Conference on Electronics and Nanotechnology (ELNANO); 2020 April 22-24; Kyiv. Kyiv: IEEE; 2020. p. 464-468. DOI: 10.1109/ ELNANO50318.2020.9088896.
4. Tymchik G, Skytsyuk VI, Klotchko TR, Popiel P, Begaliyeva K. The active surface of the sensor at a contact to the technological object. Processing of Conference Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments; 2018; Wilga. Bellingham: SPIE.digitallibrary; 2018. p. 108085G. Available from: .
5. Tymchyk GS, Skytsiouk VI, Klotchko TR, Polishchuk LK, Zahorodnii S, Mekebayev N, et al. Forces balance in coordinate system of object’s existence 3D space. Processing of Conference Photonics Applications in Astronomy, Communications, Industry, and High Energy Physics Experiments; 2022; Lublin. Bellingham: SPIE.digitallibrary; 2018. p. 124760U. Available from: https://www.spiedigitallibrary.org/conferenceproceedings-of-spie/12476/ 2659188/Forces-balance-in-coordinate-system-of-objects-existence-3D-space/10.1117/12.2659188.full#_=_.
6. Tymchik G, Vysloukh SP, Tereshchenko NF, Matvienko SN. Investigation Thermal Conductivity of Biological Materials by Direct Heating Thermistor Method. Processing of 2018 IEEE 38th International Conference on Electronics and Nanotechnology (ELNANO); 2018 April 24-26; Kyiv. Kyiv: IEEE; 2018. p. 429-34.
7. Dubolazov A, Ushenko V, Trifonyuk L, Stashkevich A, Soltys I, Ushenko Y, et al. Polarization-Singular Approach to Imaging Mueller-Matrix Polarimetry in the Differential Diagnosis of Histological Sections of Biopsy of Tumors of the Uterus and Prostate. Frontiers in Physics. 2021;9:711212. DOI: 10.3389/fphy.2021.711212.
8. Ushenko VA, Hogan BT, Dubolazov A, Piavchenko G, Kuznetsov SL, Ushenko AG, et al. 3D Mueller matrix mapping of layered distributions of depolarisation degree for analysis of prostate adenoma and carcinoma diffuse tissues. Scientific Reports. 2021;11:5162. DOI: 10.1038/ s41598-021-83986-4.
9. Ushenko VA, Hogan BT, Dubolazov A, Grechina AV, Boronikhina TV, Gorsky M, et al. Embossed topographic depolarisation maps of biological tissues with different morphological structures Scientific Reports. 2021;11(1):3871. DOI: 10.1038/s41598-021-83017-2.
10. Hogan BT, Ushenko VA, Syvokorovskaya A-V, Dubolazov AV, Vanchulyak OY, Ushenko AG, et al. 3D Mueller Matrix Reconstruction of the Optical Anisotropy Parameters of Myocardial Histopathology Tissue Samples. Frontiers in Physic. 2021;9:1-14. DOI: 10.3389/ fphy.2021.737866.
11. Ushenko VO, Trifonyuk L, Ushenko YA, Dubolazov OV, Gorsky MP, Ushenko AG. Polarization singularity analysis of Mueller-matrix invariants of optical anisotropy of biological tissues samples in cancer diagnostics. Journal of Optics (United Kingdom). 2021;23(6):064004. DOI: 10.1088/2040-8986/abf97a.

Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 2, 173, 302-310 pages, index UDC 616.441-079.4

DOI:

10.29254/2077-4214-2024-2-173-302-310