Ivanytska O. S., Bilash S. M., Lychman V. O., Toropov O. A., Avetikov D. S., Horban I. I.

APPLICATION OF PLATELET-RICHED PLASMA IN THE COMPLEX TREATMENT OF PATIENTS WITH COMPLETE DISLOCATION OF MAXILLARY TEETH

---

Show/Download

About the author:

Ivanytska O. S., Bilash S. M., Lychman V. O., Toropov O. A., Avetikov D. S., Horban I. I.

Heading:

DENTISTRY

Type of article:

Scientific article

Annotation:

Restoring the connection between the tooth and the periodontium is the primary goal of complex treatment, which involves the formation of new bone and cement, restoring the periodontal ligament and blood supply to the pulp from the surrounding tissues. The study aimed to investigate the effect of platelet-rich plasma as a medium for temporarily storing a dislocated tooth on the regeneration and reconstruction of its tissues after replantation of an extracted tooth compared to saline and water. In this study, 30 adult male Wistar rats were used, and a tooth was extracted and then replanted. A fibroblast cell activity analysis was performed after 72 hours to determine cell viability. Cells incubated in platelet-rich plasma medium had a higher percentage of cell viability (82.7±5.1), followed by saline (74.3±4.2) with statistical significance (p>0.001). The minimum percentage was recorded in cells in the medium with tap water (68.8±3.9). The pulp, dentin and cementum of the group where the teeth were placed in platelet-rich plasma showed normal histological features, demonstrating optimal tissue remodelling and attachment to the surrounding bone. There were no signs of root resorption. In summary, this study used several temporary storage procedures and evaluated changes in histological events that occurred during the storage of extracted teeth and replantation until the time of recording of the success of the replantation. The study's results indicate the use of platelet-rich plasma as a storage medium, as it significantly preserves the viability of dental cells compared to water and saline solutions.

Tags:

Bibliography:

1. Shah SJ, Alshahrani I, Yaqoob A, Togoo RA, Alnazeh AA, Khan MI. Unique tooth dislocation into submental space during a pan facial trauma - Importance of an orthopantomogram. Trauma Case Rep. 2020;27:100304. DOI: 10.1016/j.tcr.2020.100304.
2. Kadulkar N, Kataki R, Deka A, Thonai S. Replantation of an Avulsed Tooth: A Case Report. Cureus. 2023;15(5):e39198. DOI: 10.7759/cureus.39198.
3. Moule AJ, Moule CA. The endodontic management of traumatized permanent anterior teeth: a review. Aust Dent J. 2007;52(1):S122-37. DOI: 10.1111/j.1834-7819.2007.tb00520.x.
4. Brandini DA, Amaral MF, Debortoli CVL, Panzarini SR. Immediate tooth replantation: root canal filling for delayed initiation of endodontic treatment. Braz Oral Res. 2018;32:e7. DOI: 10.1590/1807-3107/2018.vol32.0007.
5. Avetikov DS, Lokes KP, Ishchenko VV. Zminy mineralʹnoho komponentu nyzhnʹoshchelepnoyi kistky v dynamitsi reparatyvnoho osteohenezu za umov khronichnoyi nitratnoyi intoksykatsiyi. Visnyk problem biolohiyi i medytsyny. 2014;2.1(107):37-39. [in Ukrainian].
6. Khattou VV, Ivanytska OS, Havryliev VM, Buhanchenko OP, Boyko IV, Lokes KP, et al. Dynamics of bone formation and osteoresorption markers in patients with postoperative mandibular bone defects. World of Medicine and Biology. 2023;4(86):167-170. DOI: 10.26724/2079-8334-2023-4-86-167-170.
7. Najeeb S, Al-Quraini AAA, Almusallam HAA, Zafar MS, Khurshid Z. Effect of laser treatment on outcomes of tooth replantation - A systematic review. J Taibah Univ Med Sci. 2020;15(3):169-176. DOI: 10.1016/j.jtumed.2020.03.008.
8. Lee DJ, Lee SJ, Lee MJ, Kim EJ, Ohshima H, Jung HS. The role of angiogenesis and pulpal healing in tooth replantation and allograft transplantation. Biochem Biophys Rep. 2021;26:100945. DOI: 10.1016/j.bbrep.2021.100945.
9. Martínez-Martínez A, Ruiz-Santiago F, García-Espinosa J. Platelet-rich plasma: myth or reality? Radiologia (Engl Ed). 2018;60(6):465-475. DOI: 10.1016/j.rx.2018.08.006.
10. Xu J, Gou L, Zhang P, Li H, Qiu S. Platelet-rich plasma and regenerative dentistry. Aust Dent J. 2020;65(2):131-142. DOI: 10.1111/adj.12754.
11. Zotti F, Albanese M, Rodella LF, Nocini PF. Platelet-Rich Plasma in Treatment of Temporomandibular Joint Dysfunctions: Narrative Review. Int J Mol Sci. 2019;20(2):277. DOI: 10.3390/ijms20020277.

Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 1, 172, 491-496 pages, index UDC 616.314/.716.1-001-085:616.15

DOI:

10.29254/2077-4214-2024-1-172-491-496