Drehval O. A., Lesychna A. V., Drehval I. V., Sklyar T. V.

INFLUENCE OF CARBON AND NITROGEN SOURCES ON BIOMASS YIELD AND FUNGISTATIC ACTIVITY OF TRICHODERMA VIRIDE KMB-F-15

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About the author:

Drehval O. A., Lesychna A. V., Drehval I. V., Sklyar T. V.

Heading:

MICROBIOLOGY

Type of article:

Scientific article

Annotation:

Micromycetes of the Trichoderma genus are the most common biological agents used in agriculture today to control plant mycoses. Using biological products allows us to produce environmentally friendly agricultural products and reduce the chemical burden on the environment. When choosing carbon and nitrogen sources as a basic base for developing the optimal composition of the nutrient medium for deep cultivation of microorganisms, it is necessary to consider their genus and strain characteristics. In this study, we investigated the effect of carbon and nitrogen sources on the biomass accumulation and fungistatic activity of Trichoderma viride strain KMB-F-15, an antagonist of a wide range of phytopathogenic fungi. The fungistatic activity was determined by inhibition of growth of the phytopathogenic fungus Fusarium culmorum IMBF-50716 when the filtrate of T. viride KMB-F-1 culture fluid was added to the dense medium. It was found that glycerol and green molasses at a concentration of 20 g/l resulted in the highest yield of dry biomass of T. viride KMB-F-15 (5.0 g/l and 4.9 g/l, respectively). The most favourable nitrogen sources for the fungus growth at a concentration of 5 g/l were yeast autolysate (dry biomass yield – 4.4 g/l) and ammonium chloride (3.3 g/l). The fungistatic activity of T. viride KMB-F-15, regardless of the carbon source, was high (94.7-100%). The manifestation of fungistatic activity was influenced by the source of nitrogen nutrition. The highest percentage of growth inhibition of the phytopathogen was observed when corn extract, yeast autolysate, L-glutamic acid, ammonium chloride or ammonium sulfate were used (88.5-100%).

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Bibliography:

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Publication of the article:

«Bulletin of problems biology and medicine», 2024 Issue 1, 172, 363-371 pages, index UDC 579.264 +579.64

DOI:

10.29254/2077-4214-2024-1-172-363-371