Rud M. V., Shepitko V. I., Stetsuk Ye. V., Akimov O.Ye.

MORPHOLOGY

Scentific article

Macrophages play a central role in both tissue homeostasis and inflammation. Understanding the origin, development and involvement of tissue-resident macrophages in the regulation of homeostatic processes is fundamental to the development of future intervention strategies to modulate macrophage functions in specific areas. Resident liver macrophages include Kupffer cells, which together with sinusoidal cells and Ito cells form a significantpopulation of non-parenchymal, antigen-presenting cells. The androgen / androgen receptor (AR) interaction plays a role in the development of the liver in the embryonic stage, the maximum dimorphism of the effects of androgen / AR occurs after puberty due to the activity of the hypothalamic-pituitary-gonadal axis. Quercetin effect on the inflammatory process is explained by the blockade of the lipoxygenase pathway of arachidonic acid metabolism, reduced synthesis of leukotrienes. The aim of our study was to determine the qualitative and quantitative changes in Kupffer cells during chemical castration of male rats of central origin, which was caused by the introduction of a solution of triptorelin acetate. And also to elucidate the results of the quercetin action on antigen-presenting liver cells on the background of the effects caused by the use of triptorelin solution. The experiments were performed on 30 adult male white rats. Rats were divided into 3 groups: control (10), experimental 1 (10) and experimental 2 (10). Animals from experimental group 1 were injected subcutaneously with triptorelin acetate at a dose of 0.3 mg of active substance per kg of body weight. Animals from experimental group 2 received triptorelin acetate in the same dosage and quercetin 100 mg per kg body weight 3 times a week. Changes in the liver of animals from group 1 occur on cellular and subcellular levels, causing minor venous stasis, erythrocyte sludge and macrophage infiltration. Concomitant oral administration of quercetin minimizes structural and morphological changes in rat liver tissue by increasing the antioxidant protection of liver tissue.

resident macrophages, liver, triptorelin acetate, quercetin, rats.

1. Ginhoux F, Guilliams M. Tissue-Resident Macrophage Ontogeny and Homeostasis. Immunity. 2016 Mar 15;44(3):439-49. DOI: 10.1016/j. immuni. 2016.02.024.
2. Scott CL, Zheng F, De Baetselier P, Martens L, Saeys Y, De Prijck S, et al. Bone marrow-derived monocytes give rise to self-renewing and fully differentiated Kupffer cells. Nat Commun. 2016 Jan 27;7:10321. DOI: 10.1038/ncomms10321.
3. Bonnardel J, T’Jonck W, Gaublomme D, Browaeys R, Scott CL, Martens L, et al. Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche. Immunity. 2019 Oct 15;51(4):638-654.e9. DOI: 10.1016/j. immuni.2019.08.017.
4. Waxman DJ, O’Connor C. Growth hormone regulation of sex-dependent liver gene expression. Mol Endocrinol. 2006 Nov;20(11):2613-29. DOI: 10.1210/me.2006-0007.
5. Ma WL, Lai HC, Yeh S, Cai X, Chang C. Androgen receptor roles in hepatocellular carcinoma, fatty liver, cirrhosis and hepatitis. Endocr Relat Cancer. 2014 May 6;21(3):R165-82. DOI: 10.1530/ERC-13-0283.
6. Albadrani GM, Binmowyna MN, Bin-Jumah MN, El-Akabawy G, Aldera H, Al-Farga AM. Quercetin protects against experimentally-induced myocardial infarction in rats by an antioxidant potential and concomitant activation of signal transducer and activator of transcription. J Physiol Pharmacol. 2020 Dec;71(6):125-7. DOI: 10.26402/jpp.2020.
7. Wang C, Ma C, Gong L, Guo Y, Fu K, Zhang Y, et al. Macrophage Polarization and Its Role in Liver Disease. Front Immunol. 2021 Dec 14;12:803037. DOI: 10.3389/fimmu.2021.803037.
8. Krenkel O, Tacke F. Liver Macrophages in Tissue Homeostasis and Disease. Nat Rev Immunol. 2017;17(5):306-21. DOI: 10.1038/ nri.2017.11.
9. Yunna C, Mengru H, Lei W, Weidong C. Macrophage M1/M2 Polarization. Eur J Pharmacol. 2020;877:173090. DOI: 10.1016/j. ejphar.2020.173090.
10. Shapouri-Moghaddam A, Mohammadian S, Vazini H, Taghadosi M, Esmaeili S, Mardani F, et al. Macrophage Plasticity, Polarization, and Function in Health and Disease. J Cell Physiol. 2018;233(9):6425-40. DOI: 10.1002/ jcp.26429.
11. Babcock MC, DuBose LE, Witten TL, Stauffer BL, Hildreth KL, Schwartz RS, et al. Oxidative Stress and Inflammation Are Associated With Age-Related Endothelial Dysfunction in Men With Low Testosterone. J Clin Endocrinol Metab. 2022 Jan 18;107(2):e500-e514. DOI: 10.1210/clinem/dgab715.
12. Incalza MA, D’Oria R, Natalicchio A, Perrini S, Laviola L, Giorgino F. Oxidative stress and reactive oxygen species in endothelial dysfunction associated with cardiovascular and metabolic diseases. Vascul Pharmacol. 2018 Jan;100:1-19. DOI: 10.1016/j.vph.2017.05.005.
13. Yelins’ka AM, Akimov OYe, Kostenko VO. Role of AP-1 transcriptional factor in development of oxidative and nitrosative stress in periodontal tissues during systemic inflammatory response. Ukr. Biochem. J. 2019;91(1):80-5. DOI: 10.15407/ubj91.01.080.
14. Liu L, Guo H, Song A, Huang J, Zhang Y, Jin S, et al. Progranulin inhibits LPS-induced macrophage M1 polarization via NF-кB and MAPK pathways. BMC Immunol. 2020 Jun 5;21(1):32. DOI: 10.1186/s12865-020-00355-y.
15. Xu D, Hu MJ, Wang YQ, Cui YL. Antioxidant Activities of Quercetin and Its Complexes for Medicinal Application. Molecules. 2019 Mar 21;24(6):1123. DOI: 10.3390/molecules24061123.
16. Chen T, Zhang X, Zhu G, Liu H, Chen J, Wang Y, et al. Quercetin inhibits TNF-α induced HUVECs apoptosis and inflammation via downregulating NF-kB and AP-1 signaling pathway in vitro. Medicine (Baltimore). 2020 Sep 18;99(38):e22241. DOI: 10.1097/ MD.0000000000022241.
17. Dagher O, Mury P, Thorin-Trescases N, Noly PE, Thorin E, Carrier M. Therapeutic Potential of Quercetin to Alleviate Endothelial Dysfunction in Age-Related Cardiovascular Diseases. Front Cardiovasc Med. 2021 Mar 30;8:658400. DOI: 10.3389/fcvm.2021.658400.

«Bulletin of problems biology and medicine» Issue 2 Part 2 (165), 2022 year, 151-155 pages, index UDK 616.36:612.018:599.323.4:612.08

10.29254/2077-4214-2022-2-2-165-151-155