CHANGES IN THE STRUCTURE OF PERIODONTAL COMPONENTS IN THE MODELING OF ACUTE PERIODONTITIS IN RATS AND ITS CORRECTION BY STEM CELLS

  2. 2022
  3. CHANGES IN THE STRUCTURE OF PERIODONTAL COMPONENTS IN THE MODELING OF ACUTE PERIODONTITIS IN RATS AND ITS CORRECTION BY STEM CELLS
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Publication of the article:

«Bulletin of problems biology and medicine», 2022 Issue 2 Part 2, 165,

Biguliak Н. Т., Klishch I. M.

CHANGES IN THE STRUCTURE OF PERIODONTAL COMPONENTS IN THE MODELING OF ACUTE PERIODONTITIS IN RATS AND ITS CORRECTION BY STEM CELLS

About the author:

Biguliak Н. Т., Klishch I. M.

Heading:

MORPHOLOGY

Type of article:

Scentific article

Annotation:

The aim of our research was to study peculiar features of morphological changes in the components of the periodontium in the modelled acute periodontitis and its correction by stem cells. The results of histological study of periodontal tissues of the animals with acute experimental periodontitis, which was corrected using human mesenchymal stem cells were analysed. Depending on the treatment, the animals were divided into 4 groups: the 1st group – the animals without pathology (intact); the 2nd – the animals with modelled acute periodontitis; the 3rd – the animals with periodontitis corrected by human mesenchymal stem cells. The material for histological study was obtained on the 1st, 7th, 14th and 21st days after taking the animals out of the experiment. Periodontal tissues of the animals were used as the material for morphological studies. For photo documentation, the images of histological specimens were displayed on a computer monitor using a Nicon Eclipse C microscope and a SCMOS Digital Camera by means of ToupWiev. In the experimental modelling of acute periodontitis the destructive changes in the epithelium and connective tissue stroma, impaired blood flow, inflammatory response in the gum tissue were present. According to the results of the research, it was established that correction by human mesenchymal stem cells positive effect on a course of the modeled pathological process. The morphological changes characteristic of periodontitis gradually reduced, i.e. the normal blood supply to the tissues restored, the arteries were less full-blooded, the tone of their walls decreased. Consequently, the swelling of the tissues gradually decreased becoming of original size. The polymorphonuclear infiltration fields decreased and gradually disappeared. Osteoblasts promoted restoration of the bone structure of the jaw alveolar process. The size of the periodontal pocket decreased, and in some areas it fully closed as a result of complete adhesion of the mucous membrane of the gums to the tooth, which rose on its surface upwards.

Tags:

acute experimental periodontitis, stem cells, regeneration.

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Publication of the article:

«Bulletin of problems biology and medicine» Issue 2 Part 2 (165), 2022 year, 22-31 pages, index UDK 616.314.17-002.1-091.8-085.361-092.9

DOI:

10.29254/2077-4214-2022-2-2-165-22-31

2022 Issue 2 Part 2, 165
04.02.2023
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