Kiriya D. G.
THE ROLE OF EPITHELIAL-MESENCHYMAL TRANSFORMATION IN INFERTILITY CAUSED BY CHRONIC ENDOMETRITIS
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About the author:
Kiriya D. G.
Heading:
PATHOMORPHOLOGY
Type of article:
Scientific article
Annotation:
Chronic endometritis (CE) is a fairly common pathology among women suffering from infertility and accounts for 10-25% of all cases of infertility. Epithelial-mesenchymal transformation (EMT) in СE plays a leading role in the inability of the embryo to implant in the endometrium. The purpose of the study is to improve the morphological criteria for diagnosing and predicting the course of СE. Materials and methods. To conduct a study of the role of EMT in infertility with CE, 2 research groups were formed: women before the therapy (group I) and after treatment with the subsequent onset of pregnancy and birth (group II). Immunohistochemical study was performed using monoclonal antibodies to E-cadherin, Vimentin and CD138. The results. In the group of women who underwent treatment, a significant decrease (p<0.0001) in the expression of CD138 was established in comparison with the results before the therapy. The phenomenon of EMT was observed in all cases of chronic endometritis before treatment. After therapy, the reverse process was observed – mesenchymal-epithelial transition. The level of E-cadherin after treatment was observed in the range of 51-100% (χ 2 =4.29, p=0.04) and Vimentin <10% (χ 2 =100, p<0.0001). Such levels of expression of both markers were favorable for the onset of pregnancy. An inverse correlation between the expression of CD138 and E-cadherin was detected in the I study group (p=0.011), in the II group no such connection was detected (p=0.65). Conclusions. It is possible to state the success of the treatment of XE according to the IGC study when the level of E-cadherin is 51-100% (p=0.04), and Vimentin is <10% (p<0.0001), as well as a significant decrease in the number of CD138+ cells (p <0.0001) in comparison with the primary endometrial biopsy sample. Further studies should also investigate the absolute number of CD138+ cells that are admissible after treatment.
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Bibliography:
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Publication of the article:
«Bulletin of problems biology and medicine», 2023 Issue 1, 168, 343-351 pages, index UDC 618.14-002/.177
